白藜芦醇干预缺血后处理对受损H9C2心肌细胞修复的研究

Resveratrol Affects impaired H9C2 Cells Treated With Ischemic Postconditioning

目的近年来研究发现白藜芦醇(Resveratrol,RSV)具有抗氧化、清除自由基、减轻缺血再灌注损伤等作用。将其与减轻缺血再灌注损伤的主要手段——缺血后处理(Ischemic Postconditioning,IPO)相结合,共同作用于体外培养的H9C2心肌细胞受损模型。方法采用免疫细胞化学、western blot以及RT-PCR等方法,观察这种共同作用对体外培养的H9C2心肌细胞受损模型细胞增殖的影响。结果 p38表达量随着RSV+IPO的干预和时间的延长而增加;通过直观和检测cyclinA2发现H9C2细胞数量因加入干预手段有所增加,cyclinA2的表达量也有相同趋势。结论研究结果表明,该方法不仅可以抑制细胞凋亡,更具有促进细胞增殖的作用。

Objective Recent studies have found that resveratrol （RSV） has antioxidant, free radical scavenging, reducing the role of ischemia-reperfusion injury. In this study the resveratrol and Ischemic Postconditioning （IPO） were co-administered through the impaired H9C2 cells. Methods To test the ability of the RSV and IPO to promote the H9C2 cells proliferation, immunofluorescence technique, western blot and RT-PCR were used. Results The expression of p38 which was relevant to the intervention of RSV＋IPO increased with the time. In the paper we have found the number of H9C2 cells raised under the intervention by detecting cyclinA2, and so did cyclinA2. Conclusion These results demonstrated that resveratrol affecting impaired myocardial infarction treated with ischemic postconditioning can inhibit cell apoptosis, and have an important role of promoting the proliferation.