晶状体吸出术后兔眼后囊膜组织MMP-2、TIMI-2的表达及意义

Expression of MMP-2 and TIMP-2 in the posterior capsule after aspiration of the lens in rabbits

目的观察透明晶状体皮质吸出术后早期兔眼后囊膜组织基质金属蛋白酶-2(MMP-2)及其组织抑制因子(TIMP-2)的表达变化,并探讨其意义。方法 40只成年新西兰健康大白兔,随机分为5组,各8只。每兔一眼行晶状体吸出术,另一眼作对照(不接受任何手术)。分别于术后第1、3、7、14及28天行空气栓塞法各处死一组动物,立即摘出眼球,甲醛固定,免疫组织化学法检测MMP-2、TIMP-2,随机取一组的对侧眼做为对照组。结果对照组眼后囊膜有低的MMP-2、TIMP-2表达;术后第1天术眼晶状体后囊膜即可见MMP-2、TIMP-2表达增强,术后第3天、第7天较第1、3天增强;术后第14天后囊膜上成纤维细胞成簇状排列,其胞质内可见强的MMP-2、TIMP-2表达,赤道部残留上皮细胞胞质内亦见MMP-2、TIMP-2强表达;术后第28天,瞳孔区后囊膜存留细胞胞质内见MMP-2、TIMP-2强表达。结论晶状体吸出术后早期兔眼后囊膜组织MMP-2、TIMP-2表达升高,在后囊膜混浊及纤维化的始动阶段可能起了重要作用。

【Objective】 To study the expression of matrix metalloproteinase-2（MMP-2） and its specific tissue inhibitor（TIMP-2） in the tissue of the posterior capsule of the rabbits.【Methods】 Forty adult rabbits were adopted and divided into 5 groups,8 rabbits to each group.in this study.In each rabbit,one eye undergone aspiration of the lens and the other eye as normal controls.The samples of the posterior capsule were collected on the postoperative day 1,3,7,14 and 28 respectively,the immunohistochemical methods were employed to check the expression of MMP-2/TIMP-2 on the posterior subcapsule,randomly taking 1 group of normal eyes as control.【Results】 There was weak expression of MMP-2 and TIMP-2 in the normal lens epithelial cells.There was more expression of MMP-2 and TIMP-2 in the posterior capsules after aspiration of the lens,the expression was stronger with time went.【Conclusion】 MMP-2/TIMP-2 may play important role in the posterior capasular opacification and fibrosis.