橙皮甙对人非小细胞肺癌A549/DDP细胞增殖的影响

Effect of the hesperidin on the proliferation of human non-small cell lung cancer cell line A549/DDP

目的观察橙皮甙对耐顺铂的人非小细胞肺癌A549/DDP细胞增殖的影响。方法培养A549及A549/DDP细胞,绘制细胞生长曲线;MTT法测定A549/DDP细胞对顺铂的耐药倍数;②测定6、12、24、48及96 mg/L橙皮甙在6、12、24及48 h对A549/DDP细胞增殖的抑制率。③用24 mg/L橙皮甙处理A549/DDP细胞24 h后,用流式细胞计量术分析细胞周期。结果①A549/DDP细胞和A549细胞的生长曲线相似,倍增时间分别为(33.68±0.11)h和(33.54±0.84)h;A549/DDP细胞对顺铂的耐药倍数为12.90。②橙皮甙对A549/DDP细胞的抑制率随橙皮甙浓度增加和(或)时间的延长而增强(Pearson相关分析P<0.05)。③24 mg/L橙皮甙作用A549/DDP细胞24 h后,实验组G2/M期和S期细胞数比对照组减少(P<0.05)。结论①A549/DDP细胞基本保留了A549细胞的生长特性,对顺铂有明显耐药性。②橙皮甙对A549/DDP生长有抑制作用。③橙皮甙可诱导A549/DDP细胞阻滞于G2/M期。

【Objective】 To explore anti-proliferative effects of hesperidin on human non-small cell lung cancer cell line A549/DDP.【Methods】 Growth curves of A549 and A549/DDP cell lines were plotted,and MTT assay was applied to evaluate the resistance index of A549/DDP cell line to cisplatin.Inhibition rate of A549/DDP cell proliferation was evaluated by MTT assay after treatment 6,12,24 and 48 h with 6,12,24,48 and 96 mg/L hesperidin respectively.A549/DDP cell cycle analysis was detected by flow cytometry 24 h after hesperidin treatment at the concentration close to IC50.【Results】 The cell growth curve of A549/DDP was similar to that of A549.The doubling generation time of A549 and A549/DDP were（33.68±0.11） h and（33.54±0.84） h,respectively.After 48 h treatment with cisplatine,IC50 values of cisplatine to A549 and A549/DDP were 12.68 μmol/L and 163.54 μmol/L respectively.Consequently,the resistance index of A549/DDP to cisplatine was defined as 12.90.A549/DDP proliferations were dose-and time-dependently inhibted（P 0.05） following treatment with hesperidin.After 24 h treatment with 24 mg/L of hesperidin,there is a pronounced decrease of the cell rate in S phase,along with a significant arrest in G2/M phase.【Conclusions】 A549/DDP has similar growth property with A549.A549/DDP is more resistant to traditional chemotherapeutics cisplatin.Hesperidin may inhibit A549/DDP proliferation with dose-and time-dependently.Hesperidin may cause A549/DDP arrest in G2/M phase.