子宫内膜异位症患者腹腔液及血清中干细胞生长因子的测定及意义

Evaluation of stem cell factor levels in peritoneal fluid and serum of women with endometriosis

目的:通过测定子宫内膜异位症(EMs)患者腹腔液及血清中干细胞生长因子(SCF)水平,初步探讨SCF在EMs发病机制中的作用。方法:选择经手术治疗并经病理诊断证实的EMs患者35例(异位症组),同期因不孕或卵巢囊肿行腹腔镜手术的患者30例作为对照组,采用酶联免疫吸附方法(ELISA)测定两组患者腹腔液及血清中SCF水平。结果:异位症组腹腔液SCF水平明显高于对照组(P<0.01)。异位症组血清SCF水平高于对照组,但差异无显著性(P>0.05)。异位症组腹腔液SCF水平明显高于血清值(P<0.01)。对照组腹腔液SCF水平略高于血清值,但差异无显著性(P>0.05)。异位症组Ⅰ期和Ⅱ期患者腹腔液SCF水平明显高于Ⅲ期和Ⅳ期患者(P<0.01),而Ⅲ期和Ⅳ期患者腹腔液SCF水平明显高于对照组。两组患者腹腔液及血清SCF水平分泌期及增殖期差异均无显著性(P>0.05)。结论:EMs患者腹腔液SCF水平升高,提示SCF可能在EMs发病过程中发挥一定作用。

Objective To investigate the levels of stem cell factor （SCF） in peritoneal fluid （PF） of women with endometriosis, and explore the possible involvement of SCF in the pathogenesis of endometriosis. Methods 35 patients who were diagnosed as endometriosis by operation were taken as study group, 30 women who accepted laparoscopy due to infertility or ovarian cyst were taken as the control group. SCF levels in PF and serum collected from these two groups were measured by specific enzyme-linked immunosorbent assay （ELISA）. Results SCF levels of PF in study group were significantly higher than those in control group （P 〈 0.01 ）. But there was no significant difference of serum SCF between study group and control group. SCF levels in PF were significantly higher than that in serum of study group （P 〈 0.01 ）. For SCF levels in PF of study group stratified by disease stage, women with stage I and ]1 exhibited relatively higher SCF levels in PF, whereas SCF levels in PF with stage m and IV were higher than those without endometriosis （P 〈 0.01 ）. There was no significant difference between secretory phase and proliferative phase in both groups （P 〉 0.05）. Conclusion We demonstrated an increase of SCF levels in PF associated with endometriosis. The present results suggest that SCF might play a role in the pathogenesis of endometriosis. ｜