RP-HPLC法测定不同产地不同部位贯叶金丝桃中金丝桃苷的含量

Content Determination of Hyperoside in Different Parts of Hypericum perforatum from Different Habitats by RP-HPLC

目的:建立测定贯叶金丝桃中金丝桃苷含量的方法,并测定不同产地不同部位贯叶金丝桃中金丝桃苷的含量。方法:采用反相高效液相色谱法。色谱柱为Boston Breeze-C18(250mm×4.6mm,5μm),流动相为甲醇-0.1%磷酸水溶液(40∶60,V/V),检测波长为360nm,流速为1.0mL·min-1。结果:金丝桃苷进样浓度在0.14～2.80μg·mL-1范围内与峰面积积分值呈良好的线性关系(r=0.9994),平均加样回收率为95.97%,RSD=1.16%(n=6)。四川产地贯叶金丝桃花、叶中的金丝桃苷含量最高。结论:本方法简便、准确、重复性好,可用于贯叶金丝桃的质量控制。

OBJECTIVE：To develop the method for the content determination of hyperoside in Hypericum perforatum,and to determine the content of hyperoside in different parts of H.perforatum from different habitats.METHODS：RP-HPLC method was adopted.The determination was performed on Boston Breeze-C18（250 mm×4.6 mm,5 μm） column with mobile phase consisted of methanol-0.1% phosophoric acid（40：60,V/V） at the flow rate of 1.0 mL·min-1.The detection wavelength was set at 360 nm.RESULTS：The linear rang of hypericin was 0.14～2.80 μg·mL-1（r=0.999 4） with an average recovery of 95.97%（RSD=1.16%,n=6）.The contents of hypericin in the flowers and leaves of H.perforatum from Sichuan province were the highest.CONCLUSION：The method is simple,accurate and reproducible for the quality control of H.perforatum.