甲基硒酸抑制肾癌GRC-1细胞生长与脂质过氧化关系的研究

Effects of the Renal Cell Carcinoma Growth Inhibition of Methylseleninic Acid and the Relationship of Lipid Peroxidation

目的:研究甲基硒酸对肾癌GRC-1的生长抑制效应及细胞内活性氧、脂质过氧化指标的变化。方法:使用不同浓度的甲基硒酸(1.0μmol/L、2.5μmol/L、5.0μmol/L)对GRC-1细胞进行侵染,通过四甲基偶氮唑盐(MTT)比色法检测亚硒酸钠对人脑胶质瘤干细胞的增殖的影响,使用荧光检测仪检测GRC-1细胞内ROS含量,并使用试剂盒检测GSH、SOD、MDA的含量。结果:甲基硒酸对肾癌GRC-1细胞具有明显的抑制作用,且随甲基硒酸浓度的增加,GRC-1细胞生长抑制作用逐渐加强,各染硒组差异均有统计学意义(P<0.05);GRC-1内ROS含量在低、中均显著性增加;GSH的含量在中、高剂量组明显减少,SOD的含量在各剂量组均明显减少,MDA的含量逐渐增加在高剂量组明显增加。结论:甲基硒酸能抑制肾癌GRC-1细胞的生长,伴随着ROS及脂质过氧化指标的变化。

Objective：To investigate effects of methylseleninic acid on the GRC-1 growth inhibition and changes of intracellular reactive oxygen species and lipid peroxidation indexes.Methods：Human renal cell carcinoma cells（GRC-1） were treated with different concentrations（1.0 μmol/L、2.5 μmol/L、5.0 μmol/L）of methylseleninic acid.Cell growth inhibition of GRC-1 was detected by assay of MTT.Fluorescence probe was used to label GRC-1,intracellular reactive oxygen species were observed with a fluorescence detector device,and GSH,SOD,MDA were tested by reagent kits.Results：Methylseleninic acid can significantly suppressed the growth of GRC-1.The cell growth rate was decreasing with the dose of methylseleninic acid increasing.statistical significance were be found in the all experimental groups（P0.05）.Intracellular reactive oxygen species was increased significantly in 1.0 μmol/L and 2.5 μmol/L group（P0.05）.Content of GSH was significantly decreased in middle and high groups,content of SOD was significantly decreased in all experimental groups,content of MDA was significantly increased in high group.Conclusions：Methylseleninic acid suppressed the growth of GRC-1,with changes of ROS and lipid peroxidation indexes.