硝基苯好氧降解的共基质及生物协同作用

Bio-cooperation effect and mixing substrates in the aerobic degradation of nitrobenzene

通过研究多菌种多基质条件下硝基苯的降解以便寻找提高实际废水处理效率的途径，从不同菌源中筛选与驯化获得硝基苯的高效降解菌枯草芽孢杆菌、门多萨假单孢菌、肺炎克雷伯氏菌及人苍白杆菌.研究这些菌种好氧降解硝基苯过程中的生物协同作用及其基质效应，构建实际废水处理的生物降解体系．结果表明，枯草芽孢杆菌和人苍白杆菌之间存在协同降解作用．加入人苍白杆菌后可使质量浓度为232．0mg/L硝基苯被完全降解的时间从145h缩短为85h;由枯草芽孢杆菌、门多萨假单孢菌和肺炎克雷伯氏菌组成的混合降解实验发现，枯草芽孢杆菌和门多萨假单孢菌之间存在一定程度的相互抑制作用；当加入肺炎克雷伯氏菌后抑制作用消失，降解速度加快.复合菌的作用明显优于各菌单独作用或任何两种菌之间的配合效果.经氯霉素制药厂含硝基苯实际废水的检验，混合菌在降解硝基苯的同时也使COD成分得到有效去除．

The degradation situation of nitrobenzene under conditions of many bacteria strains and substrates was studied to find the way to enhance the efficiency of actual waste water treatment; Bacillus subilis, Pseudomonas mendocina, Klebsiella pneumoniae and Ochrobactrum anthropi which are highly effective in degrading nitrobenzene were screened and domesticated from different bacteria sources; bio-cooperation and substrate effect of these bacteria strains on aerobic degradation of nitrobenzene were investigated; and biological degradation system for actual waste wate treatment was constructed. The results show that there exists bio-cooperation degradation between Bacillus subilis and Ochrobactrum anthropi. With joining of Ochrobactrum anthropi, the complete degradation time of nitrbenzene will be deduced from 145 hto 85 h when the mass concentration of nitrobenzene is 232 mg/L. For Bacillus subilis, Pseudomonas mendocina and Klebsiella pneumoniae, mutual inhibition exists between Bacillus subilis and Pseudomonas mendocina while inhibition will be disappeared and degradation rate will be increased when Klebsiella pneumoniae is added. Furthermore, the degradation rate by combination of three bacteria is much higher than by individual effect or combination of two bacteria. In actual test of nitrobenzene-containing wastewater of pharmacy factory,the COD component can be removed at the same time when nitrobenzene is degraded under mixing strains.