针灸对帕金森小鼠模型运动机能调节的分子机制研究

Acupuncture improve motor function in a mouse model of Parkinson's disease

针灸是一种中国传统的医疗手段,它通过将针插入人体特定的部位(穴位)进行刺激从而达到治疗疾病的目的,针灸的疗效已经得到了世界广泛认可。本文重点探讨针灸对帕金森病(Parkinson’s Disease,PD)模型小鼠运动机能的影响及其分子机制,采用1-甲基-4-苯基-1,2,3,6-四氢吡啶(MPTP)制备小鼠PD模型,发现针灸阳陵泉穴(GB34)能够保护PD小鼠多巴胺神经元免受损伤,从而改善不协调的运动。对其分子机制的研究还发现了一系列有趣的现象,发现针灸可以促进溶酶体膜的修复和ɑ-synuclein毒性蛋白的降解,提示针灸有可能改善PD小鼠的自噬水平。为揭示针灸治疗的作用机理及分子机制提供了新的思路。

Acupuncture is a procedure in which fine needles are inserted into an individual at discrete points and then manipulated, with the intent of treating disease. Acupuncture is practiced worldwide, despite difficulties in reconciling its principles with evidence-based medicine. Here we studied the effect of acupuncture treatment on motor function and its underlying mechanism in mouse model of Parkinson＇s Disease （PD） using 1-methyl-4-phenyl- 1,2,3,6-terahydropyridine（MPTP）. We found that acupuncture treatment at acupoint GB34 improved motor function of model mice. We also found evidence that lysosomal membranes were repaired and ot-synuclein was degraded, suggesting that the autophagy level of PD model mice was increased. This study reveals a new possible mechanism of acupuncture treatment.