响应面法优化马血中SOD热变性提纯工艺研究

Optimization of heating denaturation extraction technique of SOD from horse blood by using response surface methodology

以SOD比活力为响应值,在单因素实验的基础上,筛选出热变温度、热变时间、Cu2+添加量3个对提纯影响较大的因素,响应面法进行优化,并验证优化方案。结果表明,SOD热变性提纯的优化参数为:通过实验,热变温度为55℃,时间为15.5min,Cu2+添加量为2.08%。在此条件下,SOD比活力为700.58U/mg。

Based on single factor experiment, the report screened out three main factors, including the heating denatureation temperature, heating denatureation time, and amount volume of Cu^2＋ at first optimization of these factors by using response surface methodology. Results of response surface analysis showed that the optimum heating denatureation extraction conditions of SOD were as follows： heating denatureation temperature 55℃, heating denatureation time 15.5 rain, amount volume of Cu2＋ 2.08%. Through this condition, specific activitv of SOD to 700.58 U/ma.