鲍氏层孔菌菌丝体多糖初步理化特性及其清除自由基活性

The preliminary physical/chemical properties and free radical-scavenging activities of mycelial polysaccharides from Phellinus baumii

为充分开发鲍氏层孔菌多糖资源,采用物理的、化学的方法对鲍氏层孔菌多糖的初步理化特性和体外清除自由基能力进行研究。结果表明,鲍氏层孔菌粗多糖的总糖、蛋白质以及糖醛酸的含量分别为(61.82±1.39)%、(1.15±0.14)%、(1.70±0.09)%;其相对分子质量分布于2.95×103ku和16.29ku;并发现鲍氏层孔多糖在0～3200μg/mL的剂量范围内,对超氧自由基、羟基自由基、脂质过氧化自由基均有一定的清除作用,是一种潜在的具有较高开发利用价值的食品和药物资源。

In order to utilize polysaccharides resources in Phellinus baumii Pilat, the preliminary physical/chemical properties and radical-scavenging activities in vitro of mycelial polysaccharides （PBMP） were studied by physical and chemical methods. As results, preliminary feature of PBMP, including polysaccharides content, protein content, uronic acid content and relative molecular weight, were determined as following： （61.82±1.39）%, （1.15±0.14）%, （1.70±0.09）%, and 2.95×103 ku and 16.29 ku, respectively. In addition, scavenging free radical activities in vitro of crude PBMP was measured by using the chemical methods. Results show that PBMP could scavenge free radicals of O2, OH. and RCOO. in dose- dependent manner and is a potential food and drug resourses with high value of development and utilization.