益母草多糖的抗氧化性

Antioxidability of Polysaccharide from Leonurus japonicus Houtt

测定益母草多糖含量和研究其多糖的抗氧化性。利用水提醇沉法提取粗多糖,并利用Sevage法脱蛋白对其进行纯化;采用苯酚-硫酸法测定益母草多糖样品中纯多糖的含量并采用邻苯三酚自氧化法和水杨酸法分别研究益母草多糖清除O2-·和·OH的效果。益母草提取所得多糖样品中纯多糖含量达48·5%,益母草多糖具有较强的清除O2-·和·OH的作用。

The content of polysaccharide in Leonurus japonicus Houtt was determined,and the antioxidability of polysaccharide was investigation. The raw polysaccharide was extracted by water extraction and alcohol precipitation method,and was purified to removal proteins by Sevage method. The content of pure polysaccharide in raw polysaccharide from Leonurus japonicus Houtt was determined by phenol-sulfuric acid method,and the scavenging effects of polysaccharide in Leonurus japonicus Houtt on O2-· and · OH were investigated by pogallol autoxidation and salicylic acid method. The content of pure polysaccharide in raw polysaccharide from Leonurus japonicus Houtt reached 48.5%, and polysaccharide in Leonurus japonicus Houtt had strong effects on scavenging O2-· and·OH.