基质金属蛋白酶-1及其组织抑制因子-1、肿瘤坏死因子-α在溃疡性结肠炎患者外周血中的表达及其意义

Expression and significance of matrix metalloproteinases-1 and its organization inhibitory factor-1,tumor necrosis factor-α in peripheral blood of patients with ulcerative colitis

目的探讨基质金属蛋白酶-1(MMP-1)及其组织抑制因子-1(TIMP-1)、肿瘤坏死因子-α(TNF-α)在溃疡性结肠炎患者外周血中的表达及其意义。方法 46例溃疡性结肠炎患者与40例健康体检者,分别设为UC组与对照组。清晨空腹采取静脉血,离心分离血浆,ELISA法检测血浆中MMP-1、TIMP-1、TNF-α水平。结果 UC组患者(活动期,缓解期)血浆MMP-1、TIMP-1、TNFα-水平明显高于对照组(P<0.05);UC组活动期MMP-1、TIMP-1、TNFα-水平明显高于缓解期(P<0.05)。不同病情UC患者血浆MMP-1、TIMP-1、TNF-α水平有显著差异(P<0.05),病情越重,血浆MMP-1、TIMP-1、TNF-α水平越高。结论外周血MMP-1、TIMP-1、TNFα-水平可反映UC病情变化。

Objective To explore the expression and its significance of MMP-1, TIMP-1 and TNF-α in patients with ulcerative colitis. Methods Forty-six patients with ulcerative colitis were included in the UC group and another 40 healthy subjects were included in the control group. Venous blood was taken in the early morning, and centrifugal separation of plasma was conducted. ELISA was employed to detect the levels of plasma MMP-1, TIMP-1 and TNF-α. Results The levels of plasma MMP-1, TIMP-1 and TNF-α in the UC group（active period, remission period） were obviously higher than those in the control group （P〈0. 05）. The levels of MMP-1, TIMP-1 and TNF-α were significantly higher in the active period than in the remission period （P 〈 0.05）. The levels of plasma MMP-1, TIMP-1 and TNF-α of patients with different disease severity showed significant differences （P 〈 0.05）. The heavier the disease was, the higher of the levels of plasma MMP-1, TIMP-1 and TNF-α were. Conclusion The levels of MMP-1, TIMP-1 and TNF-α in peripheral blood ean reflect the chanes of UC condition.