可透过血脑屏障的MRI造影剂的实验研究

An empirical study on MRI contrast agents passing through the blood brain barrier

目的探索开发一种可透过血脑屏障的MRI造影剂,为进一步开发靶向脑内的特异性分子探针打下基础。方法用聚山梨酯80包被Fe3O4纳米颗粒制成注射液,注入20只新西兰兔耳背静脉内。结果注入造影剂后第5、10、30分钟行MR扫描,20只实验兔T2WI脑实质信号随着时间延长而逐渐降低,12 h后行MR扫描,信号显著降低。结论聚山梨酯80包被的Fe3O4纳米颗粒,是一种理想的穿透血脑屏障的MRI造影剂,可用于靶向脑内的分子探针的开发。

Objective To develop a kind of contrast agent which can pass through blood brain barrier for MRI use, and to form a base to further develop the special targeted inner-brain molecular probe. Methods The mixture of Fe3O4 nanoparticles packed by polysorbate80 was inject- ed into 20 New Zealand rabbits. Results MRI scanning was carried out 5 min, 10 min and 30 min after the injection. The T2WI showed that the signal of the brain essence of the 20 rabbits dropped gradually with time passing by. And 12 hours later, the signal dropped significantly. Conclusion Fe3O4 nanoparticles packed by polysorbate 80 is an ideal and practical contrast agent which can pass through blood brain barrier for MRI use. This contrast agent can be used to develop the targeted in- ner-brain molecular probes.