固相萃取-气相色谱法检测烟叶中18种有机氯类杀虫剂残留

Determination of Organochlorine Pesticides Residue in Tobacco by Solid Phase Extraction and Gas Chromatography

[目的]建立固相萃取-气相色谱法检测烟草中18种有机氯类杀虫剂多残留检测分析方法。[方法]烟草样品经正己烷超声提取、Florisil固相萃取柱净化后,用气相色谱电子捕获检测器(ECD)进行有机氯类杀虫剂残留检测。[结果]在0.05～0.50μg/g浓度范围内,18种有机氯类杀虫剂的线性关系良好,回收率在81.82%～101.50%,相对标准偏差为1.95%～4.56%(n=6),检测限均为0.01μg/g。[结论]该方法灵敏、快速、简便,适用于烟叶中有机氯类杀虫剂多残留分析。

[Objective] The aim was to establish a determination method of organochlorine pesticides residue in tobacco by solid phase extraction and gas chromatography.[Method] After tobacco samples were extracted by n-hexane ultrasonic extraction and purified by Florisil solid phase extraction,they were detected by gas chromatography electron capture detector（ECD） and using mirex as an internal standard.[Result] Eighteen pesticides（spiked at 0.05,0.20 and 0.50 μg/g） had good linear relation,and the average recoveries of 18 pesticides ranged from 81.82% to 101.50% with the relative standard deviation（RSD） of 1.95%~4.56%;the limit of detection was 0.01 μg/g.[Conclusion] The method is sensitive,quick and easy,so it is suitable for the residue analysis of organochlorine pesticides in tobacco.