氟西汀对慢性温和应激抑郁模型大鼠海马神经干细胞的影响

Effects of fluoxetine on neural stem cells in hippocampus of chronic mild stress induced depression model rats

目的观察氟西汀对慢性温和应激抑郁模型大鼠海马神经干细胞的作用。方法 24只雄性大鼠分为对照组、模型组和氟西汀组,每组8只。采用慢性中等应激制作大鼠抑郁模型,敞箱实验法测定大鼠行为学变化,免疫组织化学技术检测巢蛋白(Nestin)表达以评价大鼠神经干细胞增殖情况。结果模型组大鼠的行为学得分较对照组明显降低,海马齿状回Nestin表达减少,与模型组比较,氟西汀治疗后大鼠的行为学得分升高,Nestin表达增加,差异均有统计学意义(P<0.05)。结论氟西汀能翻转慢性应激对大鼠行为的损害,这可能与其促进神经干细胞增殖有关。

Objective To observe the effects of fluoxetine on neural stem cells in the hippocampus of chronic mild stress induced depression model rats. Methods Twenty-four rats were divided into control group, model group and fluoxetine group, eight rats in each group. Depression model rats were induced by chronic mild stress, and behavior change was measured by open-field method. The expression of Nestin was detected by immunohistochemistry to evaluate the proliferation of hipp- ocampcal neural stem cells. Results In model group, the behavior and hippocampal Nestin expression were decreased than those in control group;but in fluoxetine group,the behavior and Nestin expression were increased compared with those in model group, the differences were significant （ P 〈 0.05 ）. Conclusion Fluoxetine can reverse the behavioral change of the depres- sion model rats, it may be related with mechanism of promoting the hippocampal nervous stem cells proliferation.