糖尿病大鼠肺组织中核因子-κB、白介素-4,6的表达及意义

Expression and significance of nuclear factor-κB,interleukin 4,interleukin 6 in rats with diabetics mellitus

目的通过检测核因子-κB、白介素-4、白介素-6在糖尿病大鼠模型肺组织中表达变化及相关性,探讨其在糖尿病肺炎症损伤中的作用。方法 50只大鼠分为对照组(n=20)和糖尿病组(n=30)通过高糖、高脂饮食加腹腔注射小剂量链脲佐菌素(strep-tozotocin,STZ)的方法,建立糖尿病大鼠模型,采用光镜及电镜观察10周、20周时对照组及糖尿病组的大鼠HE染色后的肺组织形态学改变,用Masson三色染色观察肺组织胶原沉积情况,应用免疫组织化学(immunohistochemistry)方法检测各组大鼠肺组织核因子-κB、白介素-4,6动态表达变化。结果糖尿病组大鼠肺组织结构紊乱,肺泡间隙增厚,肺间质和血管周围有炎症细胞浸润,胶原含量明显增多,糖尿病组大鼠肺组织核因子-κB、白介素-4、白介素-6表达增多。结论糖尿病大鼠肺组织出现了炎症改变,糖尿病大鼠肺炎症的发生可能与核因子-κB、白介素-4、白介素-6的表达变化有关。

Objective To investigate the expressions of nuclear factor-κB （NF-κB）, intedeukin 4 （IL-4）, interleukin 6 （IL-6） in lung tissue of diabetic rats, and to explore the correlation between their expressions and the pathogenesis of pneumonia in diabetic rats. Methods 50 rats were divided into control group （ n = 20） and diabetes group （ n = 30） , and the diabetic rat models were established by giving high glucose, high fat diet combined with peritoneal injection with low - dose streptozotoein （STZ）. The morphological changes of lung tissue by means of HE stain were observed by light microscope and electron microscope at 10 weeks,20 weeks for both groups, the collagen deposition condition of lung tissue was observed by Masson trichrome staining, and the dynamic changes of expressions of NF-κB, IL-4, IL-6 were detected by immunohistocbemistry. Results The tissue structure of lung of rats in diabetes group was deranged, pulmonary alveoli gap was thickened, with inflammatory cell infiltration in pulmonary mesenchymal and blood vessel surrounding tissue, and the content of collagen was significantly increased, as compared with that in control group and at 10 weeks （ P 〈 0.01 ）.The expression levels of NF-κB, IL-4, IL-6 were significantly increased, as compared with those in control group （P 〈 0. 01 ）. Condusion There appears pulmonary inflammation in pulmonary tissue of diabetic rats,which may be correlated with the changes of expression levels of NF-κB, IL-4, IL-6.