HCV细胞培养系统研究进展

Advances in the study of HCV cell culture models

我国丙型肝炎患病率高,目前临床使用干扰素┼利巴韦林治疗,但仅对50%的HCV基因1型患者有效,因此急需有效的治疗方法。由于HCV在被感染组织和血清中浓度较低,且病毒变异高,目前对HCV机制的研究仍处于初级阶段,至今尚未建立可靠、稳定的HCV动物模型及感染细胞系统。如能建立一个有效的体外复制系统,对研究HCV的附着、融合、内吞、入胞、复制、释放和发病机制等有重要意义。本文就近年来国内外出现的HCV体外感染细胞系统的研究新进展进行综述,重点对稳定性较好的HCV体外细胞培养模型和HCV假病毒感染颗粒加以阐述。

There is a high prevalence of HCV in China. Interferon plus ribavirin therapy is currently the main choice for clinical practice. However, only 50% of genotype 1-HCV-infected patients respond to the treatment. More effective therapeutic strategies are urgently needed. Because of the low concentration of HCV in infected tissues as well as serum and the high degree of strain variation of the virus, mechanism studies on HCV are very difficult and still at their relatively preliminary stage. The most prominent obstacle is that a reliable and stable HCV-infected animal model or cell line has not been established so far. It＇ll be of great importance to establish an in vitro HCV infection system for the study of the attachment, fusion, endocytosis, entry, replication and release of HCV and the pathogenesis. This review discusses the recent advances in the study of in vitro cell culture models for HCV infection, with emphasis on HCV grown in cell culture and HCV pseudoparticles.