基于基因表达谱的粗叶悬钩子总生物碱治疗大鼠非酒精性脂肪肝的实验研究

Experimental Study about Total Alkaloids from Rubus Alceaefolius Poir on Rats with Nonalcoholic Fatty Liver based on Gene Expression Profiles

目的探讨粗叶悬钩子总生物碱(TARAP)治疗非酒精性脂肪肝大鼠的分子生物学机制。方法制备非酒精性脂肪肝大鼠模型,HE染色观察脂肪肝病理变化;基因芯片分析各组的差异表达基因。结果 HE染色显示TARAP对非酒精性脂肪肝有明显的治疗作用;用基因芯片技术比较模型组和正常组,共筛选出221个表达有显著差异的基因;对模型组和正常组差异基因的GO分析提示非酒精性脂肪肝大鼠在免疫、炎症、胆固醇合成等方面和正常组比较有显著的差异;SOM聚类分析提示高剂量TARAP对207个上调基因的表达有抑制作用,对62个下调基因的表达有上调作用。结论 TARAP的干预治疗能改变大鼠免疫、炎症、代谢等相关基因的异常表达。

Objective To explore the molecular mechanism of nonalcoholic fatty liver（NFL） rats treated by total alkaloids from Rubus Alceaefolius Poir（TARAP）.Methods The rats were fed with high fat forage for 12 weeks to establish the model of NFL.The pathological change of hepatic tissue was observed by HE stain,and the differentially expressed genes were determined by gene chips.Results HE stain showed TARAP could lighten the steatosis of liver tissue,reduce lipid accumulations in liver tissue.Gene chips technology was used to screen 221 differentially expressed genes.GO analysis results of differentially expressed genes suggested that NFL rats in model group had significant differences in immunity,inflammation,cholesterol synthesis,etc..compared with control group.SOM cluster analysis results suggested that high dose of TARAP could inhibit the expression of 207 up-regulated genes,and up-regulate the expression of 62 down-regulated genes.Conclusion The intervention of TARAP can alter the abnormal expressed genes related immunity,inflammation,metabolism,etc...