Rab5a和APPLl在乳腺癌组织中的表达及意义

Expression and significance of RabSa and APPLI in breast cancer

目的探讨Rah5a和APPLl在乳腺癌组织中的表达及意义。方法采用免疫组织化学方法检测柏例乳腺纤维瘤组织和74例乳腺癌组织中Rab5a和APPLl的表达水平，分析其与乳腺癌转移、病理分级和人表皮生长因子受体2（HER-2）表达的相关性。结果Rah5a和APPLl在乳腺纤维瘤组织中的阳性率分别为45．O％和30．0％，在乳腺癌组织中的阳性率分别为91．9％和83．8％（均P〈0．01）。Rab5a在乳腺癌HER-2阳性组和阴性组中的阳性率分别为92．6％和90．0％（P〈0．05），APPLl在乳腺癌HER-2阳性组和阴性组中的阳性率分别为85．2％和80．0％（P〉0．05）。Rab5a和APPLl在有无乳腺癌局部转移和不同病理学分级中的表达差异无统计学意义（P〉0．05）。结论Rab5a和APPLl在乳腺癌中高表达，并与HER-2表达呈正相关；Rab5a和APPLl可能通过调节HER-2的信号传导而影响乳腺癌细胞生物学行为。

Objective To investigate the expression of RabSa and APPL1 in breast cancer and fibroma, and analyze their correlation with HER-2 expression, metastasis and development of breast cancer. Methods RabSa and APPL1 in paraffin embedded tissues of 74 breast carcinomas and 40 breast fibromas were detected by immunohistochemistry. Their relationship with metastasis, pathological grade, and HER-2 expression in breast cancer was determined by statistical analysis. Results There was no expression or low expression of RakSa and APPL1 in the breast fibroma, but the positive expression rate of RabSa and APPL1 in the breast carcinomas were 91.9% and 83.8%, respectively. No significant difference in expression of RabSa and APPL1 was found between metastatic and non-metastatic groups, and pathological grade Ⅰ/ Ⅱ and grade Ⅲ groups. But RahSa was overexpressed in HER-2-positive group compared with that in the HER- 2-negative group. Conclusions Rab.Sa and APPL1 are overexpressed in breast cancer, and are positively correlated with the HER-2 expression. These proteins may influence the growth and proliferation of breast cancer cells by HER-2 signal transduction.