TSHR和MMP-9在甲状腺乳头状癌组织中的表达及其临床意义

Clinical significances of the TSHR and MMP-9 expressions in papillary thyroid carcinoma

[目的]探讨甲状腺乳头状癌(PTC)组织中促甲状腺激素受体(TSHR)与基质金属蛋白酶(MMP-9)的表达及其与病理参数之间的关系。[方法]应用免疫组织化学(SP)法检测56例PTC,30例结节性甲状腺肿及11例正常甲状腺组织标本的TSHR和MMP-9蛋白表达。[结果]TSHR蛋白在PTC、结节性甲状腺肿和正常甲状腺组织的表达率分别为91.07%、70.00%和27.27%,三组间比较差异有显著性意义(P<0.05);TSHR在有淋巴结转移PTC组与无淋巴结转移组的表达率分别为76.92%与95.35%(P>0.05);在Ⅲ～Ⅳ期与Ⅰ～Ⅱ期的表达率分别为89.66%与92.60%(P>0.05)。MMP-9蛋白在PTC、结甲和正常甲状腺组织中表达率分别为88.37%、36.67%和0%,三组间比较差异有显著性意义(P<0.05);在有淋巴结转移PTC组与无淋巴结转移PTC组的表达率分别为92.31%与79.07%(P>0.05),在Ⅲ～Ⅳ期与Ⅰ～Ⅱ期的表达率分别为89.66%与74.07%(P>0.05)。TSHR、MMP-9的表达与患者的性别、年龄及肿瘤大小均无相关性(P>0.05)。[结论]TSHR蛋白高表达与甲状腺乳头状癌的发生有关,可能是甲状腺癌发生的一个早期标志;MMP-9高表达可能与甲状腺癌细胞侵袭和转移密切相关。

[Objective] To evaluate the expressions of thyroid stimulating hormone receptor（TSHR） and matrix metalloproteinase-9（MMP-9） in papillary thyroid carcinoma（PTC） and their clinical significances.[Method] The SP immunohistochemistry was used to detect the expressions of TSHR and MMP-9 protein in 56 cases of PTC,30 cases of nodular goiter and 11 cases of normal thyroid tissue.[Results] The positive expression rate of TSHR in PTC was significantly higher than those of nodular goiter and normal thyroid tissue（P0.05）.and the rates in PTC with lymph node metastasis and without were 76.92% and 95.35% respectively（P0.05）,The rates in stage Ⅲ-Ⅳand stageⅠ-Ⅱwere 89.66 % and 92.60 % respectively（P0.05）;The positive expression rate of MMP-9 protein in PTC was significantly higher than those of nodular goiter and normal thyroid tissue（P0.05）.and the rates in PTC with lymph node metastasis and without were 92.31 % and 79.07 % respectively（P0.05）,and the rates in stage Ⅲ-Ⅳand stageⅠ-Ⅱwere 89.66 % and 74.07 % respectively（P0.05）.Their expressions of both TSHR and MMP-9 were not related to sex,age and tumor size of the patient（P0.05）.[Conclusion] Over-expression of TSHR might be related to the development of PTC and become a early marker of the thyroid carcinoma.The over-expression of MMP-9 could be related to invasion and metastasis.