苦豆子种质资源遗传多样性的RAPD分析

Genetic diversity of Sophora alopecuroides germplasm resources based on RAPD analysis

为深入研究并充分利用野生苦豆子(Sophora alopecuroides)资源,本研究利用RAPD技术对22个苦豆子居群进行研究,从DNA水平探讨苦豆子种质资源的遗传多样性。结果表明,筛选得到的31条RAPD随机引物共检测出314条扩增DNA片段,其中多态性条带285条,多态性比率为90.76%。Nei’s遗传多样性指数(H)为0.301 5,Shannon信息指数(I)为0.453 1,遗传距离变化范围为0.114～0.709,所研究的苦豆子居群间具有丰富的遗传多样性。UPGMA聚类分析和主成分分析(PCA)结果显示,22个苦豆子居群聚为6类,不同居群间出现了一定的遗传分化。

Genetic diversity of Sophora alopecuroides germplasm resources was analyzed at DNA level by Random Amplified Polymorphic DNA （RAPD）. A total of 314 DNA hands from 22 populations in different habitats were amplified with 31 reliable RAPD primers, of which 285 DNA bands （90. 76%） were polymorphic. The Nei＇s gene diversity （H） and Shannon information index （I） were 0. 301 5 and 0. 453 1, respectively. The genetic distances ranged from 0. 114-0. 709. The results of RAPD unweighted pair- group method with arithmetic means （UPGMA） cluster and the principal component analysis （PCA） dem- onstrated that 22 S. alopecuroides populations were clustered into 6 groups, which means that the genetic differentiations exist among populations.