青稞Hva1-hv基因对小麦的遗传转化研究

Genetic Transformation of Hva1-hv Gene from Hulless Barley (Hordeum vulgare var. nudum) to Wheat(Triticum aestivum)

本研究通过根癌农杆菌介导,将克隆自强抗旱青稞品种的LEA3蛋白编码基因Hva1-hv转化到小麦品种Bobwhite中。经除草剂草丁膦筛选和分子检测,鉴定出4个稳定遗传的转基因小麦株系。结果表明,在干旱胁迫条件下,经T2代植株的RT-PCR分析表明,目的基因能在转基因植物中正常表达。采用离体叶片失水率作为衡量植物抗旱能力的指标,发现干旱胁迫条件下,4个T2代转基因小麦株系的离体叶片失水率较受体材料显著降低,证明转基因植株的抗干旱胁迫能力强于受体材料。本研究结果初步显示,Hva1-hv基因在抗旱小麦品种培育方面具有潜在应用价值。

In this study,agrobacterium-mediated transformation was undertaken to integrate Hva1-hv gene coding LEA3 protein from Tibetan hulless barley（H.nudum） to wheat variety Bobwhite（T.aestivum）.Four transgenic wheat strains from T0 to T2 generation were identified by herbicides screening and molecular identification.The result showed that the imported Hva1-hv gene was expressed in transgenic plants under drought stress by RT-PCR.Transgenic plants had significantly lower WLR（Water loss rate） of excised leaves than control under the drought stress.These results indicated Hva1-hv had potential application value in wheat breeding against drought stress.