14个‘黄山种’茶树单株的DNA指纹图谱构建

Establishment of DNA Fingerprinting of 14 Huangshanzhong

本研究以筛选出的12条ISSR引物对14个茶树单株进行PCR扩增。结果表明,共扩增出清晰、重复性好的谱带151个,其中,特异带140条,多态性比例为92.72%;应用引物IR43、IR51组合获得了每份茶树单株的特征谱带,可以有效区分出所有供试材料,建立了14份茶树单株的ISSR指纹图谱;经聚类分析,在相似性系数为0.507处,所有材料被分为4个群体。

Twelve selected ISSR primers were used in PCR amplification of 14 tested individual tea plants.The results showed that a total of 151 distinct,good repeatability bands were detected by using 12 ISSR primers,of which 140 were polymorphic,and the percentage of polymorphic bands was 92.72 %.The specific bands of each individual tea plant were obtained by applying primer IR43 and IR51,which could effectively distinguish all the materials and establish their ISSR fingerprints.Through clustering analysis,when the similarity coefficient was 0.507,all materials were divided into four groups.