大麻染色体制片技术的比较研究

Comparison of Techniques of Chromosome Preparation in Cannabis sativa L.

以大麻种子根尖和雄花花蕾为材料,采用常规压片法和去壁低渗法对大麻染色体进行观察。结果表明,去壁低渗法较常规压片法更易获得分散细胞,且染色体制片背景清晰;对大麻根尖有丝分裂不同时期进行观察,在种子发芽46 h时取材,染色体中期分裂相最多;染色体2n=20,38,40,80等不同倍性的大麻染色体细胞均有存在。

Using the conventional pressed disc method and the wall degradation hypotonic treatment,the chromosomes of male flower buds and root tips of germinated seeds of Cannabis sativa L.were studied.Compared with conventional slice-pressing method,the wall degradation hypotonic method was more effective to visualize dispersive cells for chromosomes observation,and the slides prepared by this method make it easier to get good pictures with clear background.Different mitotic phases of cells sampled from cannabis root tips were observed,and the best sampling time for chromosome observation was the 46th h after the seed germination treatment,when most of cells were at metaphase.The cells of different chromosome ploidy levels were observed with chromosome numbers 2n = 20,38,40,80.