尿孕酮酶免法对健康妇女无排卵月经周期的研究

Study of Anovulatory Menstrual Cycle in Healthy Woman by the Method of Urine Progesterone Enzyme Immunoassay

目的 实验观察 38名健康妇女连续 2～ 3个月经周期中无排卵所出现的频率。方法 收集每日第 1次晨尿 ,用酶联免疫法分别测定孕酮在尿中的代谢产物 3-葡萄糖苷酸孕二醇 (pregnanediol- 3- glucuronide,Pd G)、卵泡刺激素 (FSH)以及肌酐 (creatintine,Cr)。结果 发现无排卵人数所出现的频率为 7.90 % ,无排卵周期发生的频率为 7.6 9%。无排卵周期与正常排卵周期相比 ,FSH浓度在卵泡早期显著降低 ,孕酮的分泌始终维持在较低的水平。结论 可以用尿孕酮酶联免疫法研究卵巢功能 ,并对无排卵月经周期进行分析。

Objective In this experiment, we investigated the frequency of anovulation in a sample of 38 healthy women by collection two to three consecutive menstrual cycles.Methods Daily morning first urine samples were collected for analysis of urine progesterone (pregnanediol 3 glucuronide), FSH and creatinine by enzyme immunoassay.Results Frequency of anovulatory women was 7.90%, and anovulatory menstrual cycles frequency was 7.69%. During early follicular phase, FSH excretion was significantly less in the anovulatory cycles than ovulatory cycles, and progesterone always remained at very low level for the whole cycle.Conclusion Urine progesterone of the enzyme immunoassay can be used to evaluate ovary function and to analyze anovulatory menstrual cycle.