不同子宫内膜组织层粘连蛋白的表达及其意义

The Expression and Significance of Laminin of Three Different Endometrium

目的 观察层粘连蛋白 (laminin,L N)在正常子宫内膜、子宫内膜增殖症和子宫内膜癌中的表达规律 ,了解 L N表达与子宫内膜癌发生及发展的关系。方法 用免疫组化 L SAB法检测了 16例正常子宫内膜、12例子宫内膜增殖症、2 7例子宫内膜癌组织中 L N的表达。结果 3组内膜组织腺上皮基膜、腺细胞与间质细胞质内均有 L N表达。其中 3组内膜间腺上皮基膜 L N表达构成比 (χ2 =13.0 6 ,P<0 .0 0 1)、腺细胞 L N表达率 (χ2 =17.82 ,P<0 .0 0 1)、间质细胞 L N表达率 (χ2 =16 .95 ,P<0 .0 0 1)、腺细胞 /间质细胞 L N表达构成比 (χ2 =2 7.49,P<0 .0 0 1)比较均有显著的统计学差异 ;L N表达与内膜癌临床病理参数间比较 :内膜癌临床分期与基膜 L N表达 (P<0 .0 5 )及间质细胞 L N表达间 (P<0 .0 1) ,内膜癌病理分级与间质细胞 L N表达间 (P<0 .0 5 )有统计学差异。结论 腺上皮基膜、间质细胞 L N表达缺失 ,腺细胞内 L

Objective To investigate the expression of laminin(LN) on normal endometrium, hyperplastic endometrium and endometrial adenocarcinoma and; to study the relationship between LN′s expression and endometrial adenocarcinoma.Methods LSAB immunohistochemical method was used to study LN′s expression on 16 normal, 12 hyperplastic and 27 adenocarcinoma endometrium cases.Results Laminin is widely expressd on epithelia basement membrane(EBM), epithelial/cancer cell(EC) and stromal cell (SC). Among normal, hyperplastic, adenocarcinoma endometrium, LN′s expression on EBM (χ 2=13 06, P <0 001), EC (χ 2=17 82, P <0 001), SC (χ 2=16 96, P <0 01) and EC/SC (χ 2=27 49, P <0 001) are significantly different. The LN′s expression was compared with clinical and pathologic parameter of endometrial adenocarcinoma, and the clinical stages of endometrial adenocarcinoma compared with LN′s expression on EBM ( P <0.05) and on SC ( P <0.01),and the pathologic grades of endometrial adenocarcinoma with LN′s expression on SC ( P <0.05), all those being different in statistics.Conclusion The LN′s expression on epithelial/cancer cells and disapearane on epithelial basement membrane, stromal cells are related to endometrial carcinoma; It may be an important sign on the early stage of endometrial adenocarcinoma.