三种ED模型小鼠海绵体组织Cx43蛋白表达变化

Cx43 expression and its significance in the penile tissue of three different erectile dysfunction models of rat

目的观察三种阴茎勃起功能障碍(ED)小鼠海绵体组织中连接蛋白(Cx)43表达变化。方法将125只Wistar大鼠随机分为DM因素组35只、血管因素组31只、神经因素组28只及对照组31只,其中前三组分别通过腹腔注射链脲菌素(STZ)、结扎髂内动脉及游离并切断海绵体神经等方法建立ED模型。各组均于制模后3周进行阿朴吗啡试验,观察大鼠阴茎勃起情况;并分别于8、12、16周分批断颈处死,采用免疫组化法检测阴茎海绵体组织的Cx43。结果 DM因素组、血管因素组及神经因素组勃起比例及次数均明显低于对照组,阴茎海绵体组织Cx43蛋白表达显著低于对照组(P<0.01),尤以DM因素组为著(P<0.05)。结论三种ED小鼠海绵体组织中Cx43蛋白表达均下降,尤以DM性ED为著。

Objective To investigate the roles of connexin43 （ Cx43 ） expression in the pathogenesis of different kinds of erectile dysfunction （ED）. Methods Total of 125 Wister rats were divided into DM factor group（ n = 35 ）, vessel factor group （ n = 31 ）, nerve factor group （ n = 28 ） and control group （ n = 31 ）, the former three groups were made experimental models of DM by injecting intraperitoneally with streptozotocin（STZ） , ligaturing internal iliac artery , dissociating and cut- ting off the cavernous nerves. Penile erection of the rat models were observed by apomorphine test 3 weeks after the mould- ing;The CX43 expression was examined with immunohistochemistry method, on the 8 th, 12 th and 16 th week from the 4 th day of STZ injection. Results The ratio and frequency of erection in the former three groups were significantly lower than those in the control group, the Cx43 expression level was significantly lower than that in the control group（ P 〈 0.01 ）, especially in the DM factor group （ P 〈 0.05 ）. Conclusions The decrease of Cx43 expression play important roles in ED caused by different factors , especially in DM-associated ED.