BNIP3基因甲基化与早期结直肠癌患者临床病理特征的关系

Relationship between BNIP3 gene methylation and clinicopathological characteristics in patients with early-stage colorectal cancer

目的分析早期结直肠癌组织中BNIP3基因启动子区CpG岛甲基化与患者临床病理特征的关系,评价其在结直肠癌发生发展中的作用。方法用甲基化特异性PCR技术分析107例早期结直肠癌患者肿瘤组织中BNIP3基因启动子区CpG岛甲基化状态。结果本组结直肠癌组织标本中BNIP3基因启动子区CpG岛甲基化阳性率为58.9%(63/107)。右半结肠组肿瘤组织中BNIP3基因甲基化明显高于左半结肠组(P<0.001);低分化组明显高于高中分化组(P=0.002)。BNIP3基因甲基化在患者性别、年龄、肿瘤大小和分期等分组间无明显差异。结论早期结直肠癌组织中存在BNIP3基因启动子区CpG岛甲基化。BNIP3基因高甲基化与早期结直肠癌部位和分化程度密切相关。

Objectives To detect the DNA methylation status of the CpG island in BNIP3 gene promoter in patients with early-stage colorectal cancer,and to determine its relationship with clinicopathological features and its role in the development and progress of colorectal cancer.Methods Methylation specific PCR,DNA methylation status of the BNIP3 gene was analyzed in 107 patients with early-stage colorectal cancer.Results Hypermethylation of the CpG island in BNIP3 gene promoter was detected in 63 patients（63/107,58.9%）.The aberrant methylation of the BNIP3 gene was more common in the right colon cancer group than in the left colon cancer group（P0.001）.The BNIP3 methylation status was higher in the poor differentiated group than in the well and mediate differented group（P=0.002）.There was no association between BNIP3 gene methylation and gender,age,tumor size,or stage.Conclusions There was aberrant methylation of the BNIP3 gene in early-stage colorectal cancers.Hypermethylation of the CpG island in BNIP3 gene promoter correlates with both tumor location and differentiation status in patients with early-stage colorectal cancer.