镉对大鼠大脑皮质神经细胞Bcl-2,Bax和Caspase-3 mRNA转录的影响

Influence of cadmium on Bcl-2,Bax and Caspase-3 mRNA transcription in rats primary cultured cerebral cortical neurons

为研究镉对大鼠大脑皮质神经细胞凋亡相关基因Bcl-2、Bax和Caspase-3mRNA转录水平的影响,选用不同浓度(0、5、10、20μmol/L)醋酸镉染毒大鼠大脑皮质神经细胞12h,用MTT法检测细胞存活率,实时荧光定量PCR检测Bcl-2、Bax和Caspase-3mRNA的转录水平,并计算Bcl-2/Bax的比值。结果表明,与对照组相比,各染毒组细胞存活率随染毒剂量增加而显著下降(P<0.05或P<0.01);Bax和Caspase-3mRNA转录水平极显著升高(P<0.01),而Bcl-2mRNA转录水平显著降低(P<0.05),Bcl-2/Bax极显著降低(P<0.01)。说明镉能抑制大鼠大脑皮质神经细胞的生长,并可调节凋亡相关基因Bcl-2、Bax和Caspase-3mRNA的转录。

To investigate the effects of cadmium on transcription levels of apoptosis-related genes Bcl-2,Bax and Caspase-3 mRNA in primary cerebral cortical neurons of SD rats,the neurons were exposured to cadmium at different concentrations （0,5,10,20μmol/L ） for 12 h. MTT assay was applied to test the cell viability,and real-time fluorescent quantitative PCR was used to detect the transcription levels of Bcl-2,Bax and Caspase-3 mRNA. In comparison with the control group, the results showed that the cell viability decreased significantly with the higher concentration cadmium （P〈0.05 or P〈0.01）. Additionally, transcription levels of Bax and Caspase-3 mRNA increased significantly （P〈0.01）,but transcription level of Bcl-2 mRNA and Bcl-2/Bax decreased signifi- cantly （P〈0. 05,P〈0. 01）. It was concluded that cadmium can inhibit the growth of neurons and regulate the transcription of apoptosis-related genes,including Bcl-2,Bax and Caspase-3.