维生素D_3对人乳腺癌细胞株表皮生长因子受体mRNA表达的影响

Modulating effect of vitamin D_3 in vitro on EGFR mRNA expression of human breast cancer cell lines

目的 探讨维生素D3 对人乳腺癌细胞株细胞增殖等生物学行为的影响。方法 选用两种人类乳腺癌细胞株MCF 7及ZR 75 1,应用细胞培养及核酸分子杂交方法 ,研究使用维生素D3 以及并用三苯氧胺后 ,上述细胞株生长及表皮生长因子受体 (EGFR)mRNA表达的改变。结果(10 -10 ～ 10 -7)mol/L维生素D3 对MCF 7及ZR 75 1细胞呈现明显的抑制生长作用 ,并能抑制 (10 -9～10 -7)mol/L三苯氧胺对MCF 7细胞的刺激生长作用。低浓度 (10 -10 ～ 10 -9)mol/L维生素D3 与半数抑制浓度三苯氧胺合并使用 ,可使抑制作用进一步加强 ,并使MCF 7及ZR 75 1两细胞株的EGFRmRNA降低至对照组以下。

Objective To assess the modulating effects of vitamin D 3, combined with or without tamoxifen, on cell proliferation and EGFR mRNA level of human breast cancer. Methods Two breast cancer cell lines (MCF 7 and ZR 75 1) were treated with various concentrations of vitamin D 3, alone or in combination with tamoxifen, for 6 days. Cell proliferation was assessed by MTT uptake and EGFR mRNA level by Northern blot. Results 10 -10 ～10 -7 mol/L vitamin D 3 strongly inhibited proliferation of the 2 breast cancer lines, while 10 -9 ～ 10 -7 mol/L tamoxifen stimulated proliferation of MCF 7 cell. The growth inhibitory effect on the 2 cell lines could be augmented and accompanied by a decrease in EGFR mRNA level after combined treatment with IC 50 dose of tamoxifen and 10 -10 ～10 -9 mol/L vitamin D 3.Conclusion Vitamin D 3 can be considered as a useful anti tumor drug in the treatment of breast cancer.