CD3/MIB1与CD3/CD25在B细胞淋巴瘤中的意义

The significance of CD3/MIB1 and CD3/CD25 in B cell lymphomas

目的 探讨增生性T细胞 (proliferatingTcell,PTC)在扁桃体和B细胞来源的淋巴瘤中的分布及意义。方法 应用免疫组化双标记方法检测PTC ,应用Giemsa染色检测胃MALT淋巴瘤中幽门螺杆菌 (Hp)感染情况 ,用细胞培养检测胃MALT淋巴瘤中肿瘤细胞与Hp及T细胞的关系。结果在扁桃体的正常滤泡内与滤泡性淋巴瘤的肿瘤性滤泡内几乎找不到CD3与MIB1双阳性的PTC ,PTC主要分布在滤泡外与T区。CD3表达CD2 5呈相同分布情况。低度恶性胃粘膜相关淋巴组织型淋巴瘤与Hp的感染有关。细胞培养祛除T细胞后肿瘤细胞死亡。在弥漫型B细胞淋巴瘤中 ,PTC依恶性程度呈梯度表达。结论 在扁桃体的正常滤泡内与滤泡性淋巴瘤的肿瘤性滤泡内T细胞处于G0 期 ,胃MALT淋巴瘤中肿瘤性B细胞的增生可能依赖于Hp特异性的T细胞。肿瘤细胞向高度恶性转化的过程中与T细胞活性增强有关 。

Objective To identify the distribution and significance of proliferating T cells (PTC) in normal tonsil and a group of B cell lymphomas. Methods Double immunohistochemical stainning was used to detect PTC with MIB1 expression. Giemsa stainning was used to detect Helicobacter pylori (Hp) in gastric MALT type lymphoma. Cell culture was applied to investigate the relationship among tumor cells, Hp and T cells. Results Very few T cells expressed MIB1 in the follicular centre of normal tonsil and in that of follicular lymphoma. PTC were mainly distributed in the extrafollicular and T cell zones. So did CD3 + T cells expressed CD25. In low grade primary B cell gastric MALT type lymphomas, tumor cells proliferated in vitro in response to Hp but died in the absence of T cells. PTC increased with increase in the degree of malignancy in different kinds of B cell lymphomas. Conclusion T cells in follicular centres of follicular lymphoma are not in cell cycle. Proliferation of neoplastic B cells in low grade gastric MALT type lymphomas requires Hp specific infiltrating T cells. Transformation from low to high grade lymphoma is associated with an increase in T cell activation. T cells expressing MIB1 might be related to prognosis.