乌鲁木齐地区腹泻患儿腺病毒感染的检测和分型

Detection and typing assay of adenovirus infection among children with diarrhea in Urumqi

目的:了解2011年乌鲁木齐地区腹泻患儿腺病毒(AdV)的感染情况,分析该地区AdV的基因亚型,进行AdV感染分子流行病学调查。方法:采集在新疆维吾尔自治区人民医院住院和门诊≤5岁腹泻患儿粪便标本315份,采用胶体金法检测轮状病毒(RV)抗原,采用聚合酶链反应(PCR)法对上述标本进行AdV检测,并对AdV阳性标本测序明确基因型。结果:315份粪便标本中共检出AdV阳性12例,总阳性率为3.81%,低于RV检出率26.35%,AdV和RV混合感染3例。AdV阳性标本中肠道腺病毒(EAdV,F组)Ad41占2.54%(8/315),其次是非肠道腺病毒(NEAdV)占1.27%(4/315),分别为Ad1、Ad3、Ad5、Ad7、Ad31型。12份阳性标本测序结果提示该地区AdV与参考株的核苷酸序列同源性为73%～99%。结论:乌鲁木齐地区AdV感染呈全年散发流行,未表现出明显的季节性,其主要的流行株为Ad41型。

Objective： To study the status of adenovirus （AdV） infections in children with diarrhea and its epidemiological characteristics and genotype distribution in Urumqi in 2011. Methods： A total of 315 fecal specimens of children （ ≤5 years old） with diarrhea were collected from both outpatients and inpatients in our hospital from January to December 2011. Rotavirus antigens were detected by AC- ON Rota - Adeno. Polymerase chain reaction was used to detect AdV in fecal specimens, and genotypes were determined by sequence analy- sis. Results： Among all fecal specimens, 12 （3.81%） were detected positive for AdV which was less than the rate of rotavirus （26. 35% ） . The main subtypes of AdV were enteric adenovirus （subgenus F） Ad41 with a positive rate of 2. 54% （8/315 ） , followed by non- enteric adenovirus （Adl, Ad3, Ad5, Ad7, Ad31）with a positive rate of 1.27% （4/315） , and Ad40 was not detected. All the strains shared 73% -99% nucleotide sequence identity with different reference strains. Conclusion： AdVs is detected all through the year and there is no distinctly seasonal distribution. Of them Ad41 is dominant.