宫颈癌细胞体外培养的形态学观察

Morphological observation of cervical cancer cells cultured in vitro

目的:研究宫颈癌siha细胞在体外不同培养条件下细胞形态学的改变。方法:体外培养人宫颈癌siha细胞,以终浓度为1、5、10、15μmol/L的溶血磷脂酸和终浓度为5、10、20、40 mg/L的多西环素分别干预,并设不加任何药物作为空白对照组。各组均于干预后3、5、7天行过典酸雪夫反应(PAS)染色,用倒置显微镜观察各组宫颈癌细胞的形态变化。结果:溶血磷脂酸组siha细胞随着溶血磷脂酸浓度升高及作用时间的延长,细胞增殖能力增强,细胞异形性及可塑性增加,PAS染色阳性物质生成增多并最终形成环状结构的血管拟态;而多西环素处理组随着多西环素浓度增加,细胞增殖明显受到抑制,细胞核固缩、碎裂,细胞破坏。结论:宫颈癌siha细胞的增殖能力与细胞形态学的改变密切相关,溶血磷脂酸促进siha细胞增殖及血管生成拟态的形成,而多西环素则具有相反的抑制作用,且两者作用均有浓度及时间依赖性。

Objective： To study morphological change of cervical cancer siha cells under different culture conditions in vitro. Methods ： Human cervical carcinoma siha cells were treated with lysophosphatidic acid at final concentration of 1 μmol/L, 5 μmol/L, 10 μmol/L, 15 μmol/L or doxycycline at the concentration of 5 mg/L, 10 mg/L, 20 mg/L, 40 mg/L, respectively , with no drug treatment as control group. 3 days, 5 days and 7days after the intervention, each group was examined by the snow response code acid （PAS） staining, and cell morphological changes of each group were observed under an inverted microscope. Results： With lysophosphatidic acid concentration increasing and treatment time passing, proliferation capacity of siha cells were enhanced, cell atypia and plasticity was in- creased, with PAS stain - positive substance accumulated and eventually forming vascular mimicry of ring structure. While cell proliferation was significantly inhibited and exihibited nuclear condensation, fragmentation and cell destruction in Doxycycline treatment group. Conclusion： Cervical cancer siha cell proliferation is closely related to changes of cell morphology, lysophosphatidic acid promote siha cell proliferation and the formation of vascular mimicry. While doxycycline has the opposite effect and the effects of two drug are concentration and time dependent.