摘要
目的：观察柴胡皂甙d（SSd）对人急性早幼粒白血症细胞（HL60）糖皮质激素受体（GR）mRNA表达的调节作用及对细胞生长的影响。方法：用从柴胡中提取的单体成分SSd，以3H-胸腺嘧啶（3H－TdR）掺入法和流式细胞仪观察细胞生长，用Northernblot分析SSd对GRmRNA的改变。结果：经SSd作用后，HL60细胞2H－TdR掺入率明显降低，呈时间和剂量依赖关系，SSd10μg／ml处理48h作用最明显；流式细胞仅分析细胞呈现G0／G1期阻滞现象，GRmRNA表达增加。结论：SSd可上调HL60细胞GRmRNA表达并抑制细胞生长。
To study the effect of Saikosaponin-d (SSd) on the expression of glucocorticoid receptor (GR) mRNA and cell growth in HL60 cells. Methods: Antiproliferation effects of SSd on HL6O cells were determined by M-thymidine incorporation. Cell cycle analysis was also performed by flow cytometry. GRmRNA was analyzed by northern blot analysis. Results: After 48 hours treatment with 10μg/ml SSd, M-thymidine incorporation decreased in HL60 cells and the effect was time and dose dependent. The flow cytometry analysis showed HL60 cells were arrested at G0/G1 phase. The expression of GR mRNA increased. Conclusion: SSd exhibits the inhibition effects on cell growth in HL60 cells and could up-regulate the expression of GR mRNA in HL60 cells.
出处
《中国中西医结合杂志》
CSCD
北大核心
2000年第5期350-352,共3页
Chinese Journal of Integrated Traditional and Western Medicine
基金
国家中医药管理局基金!96F016
