参附注射液抗膈肌疲劳的实验研究

Experimental Study on Anti-fatigue Effect of Shenfu Injection on Diaphragmatic Muscle

目的：研究参附注射液对兔膈肌功能的影响。方法：以跨膈压（Pdi）、膈肌肌电图（EMGdi）及膈肌诱发电位（EP）为指标，双侧膈神经刺激30min复制膈肌疲劳（DiF），于用药前及用药后记录不同刺激频率的Pdi、EMGdi及DEP，算出高／低频（H／L）比值和中心频率（Fc），数据经方差分析q检验。结果：参附注射液2ml／kg用药后30min可提高Pdi、H／L比值、Fc和DEP幅度，缩短DEP潜伏期（P＜0.05）。结论：参附注射液有抗DiF作用。

To study the effect of Shenfu injection (SFI) on function of diaphragmatic muscle in rabbits.Methods: Taking the transdiaphragmatic pressure (Pdi), diaphragmatic electromyogram (EMGdi). and diaphragmatic evoked potential (DEP) as criteria for observation, the diaphragmatic fatigue (DiF) was induced by stimtilating bilateral phrenic nerve for 30 minutes. Pdi, EMGdi and DEP induced by stimulation with different frequency (Fc) were recorded be fore and after treatment. Then the high/low (H/L) frequency ratio and central Fc were calculated and analysed by variance analysis and q test. Results: After administration of 2 ml/kg of SFI, the Pdi, H/L, Fc and amplitude of DEP were markedly increased and latent phase of DEP was shortened by the drug, in comparing with those before treatment, P <0.05. Conclusion: SFI has the effect in antagonizing diaphragmatic fatigue.