白藜芦醇抑制肺癌Lewis荷瘤小鼠肿瘤生长及其体内外抗氧化活性

Inhibitory effect of resveratrol on tumor growth in Lewis C57BL/6J and its antioxidation activity in vivo and in vitro

目的:研究白藜芦醇体内抑制肺癌瘤块生长活性以及其体内外抗氧化活性。方法:采用肺癌Lewis C57BL/6J小鼠模型评价白藜芦醇的抗肿瘤活性,并测定荷瘤小鼠血清中超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量;采用活性氧(ROS)荧光探针测定白藜芦醇对人非小细胞肺癌SPC-A-1细胞内的ROS含量,并测定培养液中的SOD活性和MDA含量。结果:白藜芦醇能显著抑制体内肺癌瘤块的生长,高、中、低剂量组的抑制率分别为(58.4±5.5)%、(46.9±17.4)%和(31.9±8.1)%。白藜芦醇能上调荷瘤小鼠和SPC-A-1细胞的SOD活性,并降低MDA含量,还降低肿瘤细胞内的ROS含量。结论:结果表明白藜芦醇能抑制体内肺癌瘤块的生长;其抗氧化作用是其抗肺癌的主要机制之一。

OBJECTIVE To study the inhibitory effect of resveratrol on the growth of non-small cell lung cancer in vivo and its antioxidant mechanism.METHODS The Lewis C57BL/6J mouse model was established to evaluate the anti-tumor activity of resveratrol in vivo,and the serum superoxide dismutase（SOD） activity and malondialdehyde（MDA） contents of tumor-bearing mice were measured.The reactive oxygen species（ROS） fluorescent probe was used to evaluate the effect of resveratrol on human non-small cell lung cancer SPC-A-1 intracellular ROS levels.The SOD activity and MDA contents in the culture medium were also measured.RESULTS Resveratrol inhibited significantly the growth of tumor masses of lung cancer in vivo.The inhibitory effects in high,middle and low-dose groups were（58.4±5.5）%,（46.9±17.4）% and（31.9±8.1）%,respectively.Resveratrol could increase SOD activity and decrease MDA content in serum of tumor-bearing mice and in culture medium of SPC-A-1 cells,moreover,it could also reduce the levels of ROS in SPC-A-1 cells.CONCLUSION Our data showed that resveratrol could inhibit the tumor growth of lung cancer in vivo and its antioxidant action may be one of the mechanisms on anti-cancer.