β-氨基丙晴对人皮肤瘢痕组织成纤维细胞增殖的影响

The effects of β-aminopropionitrile on the proliferation in cultrured human hypertrophic scar fibroblasts

目的 观察 β 氨基丙晴 (BAPN)对人皮肤瘢痕组织成纤维细胞增殖的影响。 方法 利用MTT比色分析法及流式细胞术检测BAPN不同浓度 (mg/ml)不同时间对体外培养的人皮肤成纤维细胞增殖的影响。结果 BAPN2 4 ,48,72h对瘢痕组织成纤维细胞均有抑制作用 ,且抑制率随浓度的增加和时间的延长而增强 ,而对正常皮肤组织的成纤维细胞无作用。其ID50 为 2 53mg/ml。结论 BAPN可抑制体外瘢痕组织成纤维细胞的增殖 。

Objective To observe the effects of BAPN on the proliferation in cultured human hypertrophic scar fibroblasts.Methods By means of colorimetric MTT(tetrazolium) assay and flow cytometry,we tried to find the effects of BAPN with different concentrations and different time on the proliferation in cultured human hypertrophic scar fibroblasts.Results Concentrations of BAPN 0 074,0 222,0 667,2 0,6 0mg/ml could inhibit proliferation in cultured human hypertrophic scar fibroblasts,but had no effects on cultured human normal dermal fibroblasts.Its ID 50 is 2 53mg/ml.Conclusions BAPN could inhibit proliferation in cultured human hypertrophic scar fibroblasts and was dependent on concentration and time.