精氨酸对氧化应激仔猪生长性能和血浆游离氨基酸浓度的影响

Effects of Arginine Supplementation on Growth Performance and Free Amino Acids in Plasma of Weaned Piglets under Oxidative Stress

本试验选择24头健康PIC断奶仔公猪,单笼饲养,随机分为4个处理:对照组,氧化应激组,氧化应激组+0.8%精氨酸,1.6%精氨酸+氧化应激组。在试验第8天,各试验组仔猪腹腔注射敌草快诱导氧化应激,对照组仔猪注射等量生理盐水。在应激前(0 h),应激后6、24、48 h和96 h前腔静脉采血分离血浆。结果表明:氧化应激显著降低仔猪日增重、日均采食量及饲料转化效率。在氧化应激状态下,补充1.6%Arg显著增加仔猪的日均采食量。氧化应激降低仔猪血浆精氨酸、谷氨酸、赖氨酸、蛋氨酸、苏氨酸的浓度,显著增加血浆异亮氨酸和缬氨酸的浓度。在氧化应激条件下,补充精氨酸能显著提高血浆精氨酸、赖氨酸、异亮氨酸的浓度,显著降低血浆蛋氨酸、谷氨酸、缬氨酸的浓度。

The present study was conducted to test the effects of arginine （Arg） supplementation on growth performance and free amino acids in plasma of weaned piglets under oxidative stress. A total of twenty-four PIC weaned piglets were individually penned and randomly allocated into four groups with six replicates per group. Piglets were fed basal diet＋injected sterile saline （control group）, fed basal diet＋injected diquat （Oxidative stress group）, fed basal diet supplemented with 0.8% Arg＋injected diquat （0.8% Arg group）, and fed basal diet supplemented with 1. 6% Arg ＋ injected diquat （1. 6% Arg group）, respectively. On day 8, pigs were injected intraperitoneally either with diquat or sterile saline. Blood samples were collected before injection （Oh） and at 6, 24, d8 and 96 h post-injection for analysis. The results showed that oxidative stress significantly decreased ADG（P〈0.001）, ADFI（P〈0.001） and increased F/G of weaned pigs （P〈0.05）. However, supplementation of 1.6% Arg significantly increased ADFI of weaned pigs under oxidative stress （P〈0.05）. Oxidative stress induced by diquat significantly decreased the concentrations of arginine, glutamic acid, lysine, methionine and threonine in plasma, increased the concentrations of isoleucine and valine in plasma. Supplementation of arginine significantly increased the concentration of lysine and valine under oxidative stress. It is concluded that arginine can alleviate the growth depression and stress response induced by oxidative stress to some degree through depress the decreasing of food intake, improving amino acid level in circulation in piglets.