摘要
目的:优选丁香中抑菌成分的提取工艺。方法:以糠秕马拉色菌为供试菌,通过管碟法测试抑菌效果,通过单因素试验和正交试验确定丁香提取物的最优提取工艺。结果:丁香最优提取工艺为70%乙醇回流提取,料液比1∶25,提取时间2 h,提取温度65℃。丁香醇提物对糠秕马拉色菌的抑菌圈直径为(23.08±0.46)mm,抑菌活力为高度敏感。结论:优选的提取工艺稳定可行。
Objective： To optimize of extraction technology of antibacterial substances from Eugenia caryophyllata. Method： With malassezia furfur as test bacteria, antibacterial effect was tested by cup-plate method, extraction technology of extract from E. caryophyllata was optimized by single factor test and orthogonal test. Result： Optimum extraction technology was as following： refluxing extracted 2 h with 25 times the amount of 70% ethanol at 65℃. diameter of inhibition zone was （23. 08±0. 46） mm, antibacterial activity was hypersensitive. Conclusion： This optimized technology was stable and feasible.
出处
《中国实验方剂学杂志》
CAS
北大核心
2012年第22期68-71,共4页
Chinese Journal of Experimental Traditional Medical Formulae
关键词
丁香
糠秕马拉色菌
提取
抑菌
Eugenia caryophyllata
malassezia furfur
extraction
antibacterial
