痹通药酒中新乌头碱、次乌头碱、乌头碱的含量

Determination of Mesaconitine,Hypaconitine,Aconitine in Bitong Medicated Wine

目的:建立痹通药酒中新乌头碱、次乌头碱、乌头碱的HPLC含量测定方法。方法:Agela Promosil-C18(4.6 mm×250 mm,5μm)色谱柱,柱温35℃,流动相乙腈-四氢呋喃(25∶15)为流动相A,以0.1 moL.L-1醋酸铵溶液(每1 000 mL加冰醋酸0.5 mL)为B,梯度洗脱,流速1.0 mL·min-1,检测波长235 nm。结果:新乌头碱在0.118 9～0.990 9μg呈良好的线性关系(r=0.999 9),平均加样回收率98.07%,RSD1.7%;次乌头碱在0.074 5～0.621 0μg呈良好的线性关系(r=0.999 9),平均加样回收率99.08%,RSD1.5%;乌头碱在0.009 4～0.078 3μg呈良好的线性关系(r=0.999 9),平均加样回收率98.32%,RSD 2.0%。结论:本方法稳定易行、重复性好,可用于痹通药酒质量评价。

Objective： To establish an HPLC method for determination of mesaconitine, hypaconitine, aconitine in Bitong medicated wine. Method： A Agela Promosil-Cls （4.6 mm × 250 mm, 5 μm） column was adopted, the mobile phase consisted of acetonitrile-tetrahydrofuran （25 ： 15, phase A） , 0. 1 mol .L-1 ammonium acetate （containing 0.5 mL glacial acetic acid per 1 000 mL, phase B） with gradient elution at the flow rate of 1.0 mL .min- 1, and the detection wavelength was at 235 nm. Result： The linear range of mesaconitine was 0. 118 9- 0.990 9 μg （r = 0. 999 9）. The average recovery was 98.07% with RSD of 1.7%. The linear range of hypaconitine was 0. 074 5-0. 621 0μg （r = 0. 999 9）. The average recovery was 99.08% with RSD of 1. 5%. The linear range of aconitine was 0. 009 4-0. 078 3 μg （r =0. 999 9）. The average recovery was 98.32% with RSD of 2.0%. Conclusion： The developed method was reliable, and can be used for the quality control of Bitong medicated wine.