重庆后山栀子和国内其他产区栀子主要药效成分含量对比

Comparison of Main Effective Constituent Content of Gardenia jasminoides in Chongqing Hou-shan and Other Domestic Areas

目的:采用超高效液相色谱法测定与比较重庆后山栀子和国内其他产区栀子中栀子苷和西红花苷-1的含量。方法:采用Waters ACQUITY UPLC BEH C18(2.1 mm×50 mm,1.7μm)色谱柱,流动相甲醇-0.2%磷酸水溶液,梯度洗脱,检测波长238,440 nm,流速0.3 mL·min-1,柱温30℃。结果:栀子苷在0.008 1～1.300 g·L-1内呈良好线性(r=0.999 9),平均回收率99.6%(RSD 1.24%);西红花苷-1在0.008 7～1.392 g·L-1呈良好线性(r=0.999 9);平均回收率101.0%(RSD1.9%)。结论:该方法简便可靠,可为重庆后山栀子的品质评价与进一步开发利用提供科学的参考依据。

Objective： To determine and compare the main effective constituent （geniposide and crocin-1 ） content of Gardenia jasminoides in Chongqing Hou-shan and other domestic by ultra-performance liquid chromatography （UPLC）. Method： The samples were separated at 30℃ on a Waters ACQUITY UPLC BEH C18（2. 1 mm ×50 mm, 1.7 μm） column eluted with methanol and water containing 0.2% phosphate acid as mobile phases in a linear gradient mode. Flow rate was set at 0.3 mL -min-1 and the detection wavelength were set at 238 nm and 440 nm. Result： The calibration curve of geniposide was linear within the range from 0. 008 1 to 1. 300 g-L-1 （r =0. 999 9） and the average recovery was 99.6% （RSD 1.24% ）. The calibration eurve of crocin-1 was linear within the range from 0. 008 7 to 1. 392 g .L-1 （r =0. 999 9） and the average recovery was 101.0% （RSD 1.9% ）. Conclusion： The developed method was proved to be simple, reliable and useful by analysis of the samples from different areaS, and the experimental result could provide scientific reference for quality evaluation and offer basis for further exploitation and utilization of G. jasminoides in Hou-Shan area, Chongqing Province.