HPLC测定丹膝颗粒中丹酚酸B的含量

Determination of Salvianolic Acid B in Danxi Granula by HPLC

目的:建立测定丹膝颗粒中丹酚酸B的含量。方法:采用高效液相色谱法,Agilent Eclipse XDB-C18色谱柱(4.6mm×150 mm,5μm),甲醇-1%甲酸溶液(40∶60),检测波长286 nm,流速1.0 mL·min-1,柱温30℃。结果:丹酚酸B在0.207～8.264μg与其峰面积线性关系良好(r=0.999 9),平均回收率分别为100.20%,RSD 1.14%。结论:方法专属性强、重复性好、结果准确可靠,可用于丹膝颗粒的质量控制和评价方法。

Objective： To establish an HPLC method for determination of salvianolic acid B in Danxi Granula. Method： The Agilent Eclipse XDB-C18（4.6mm×150mm,5μm） was used; the flow rate was 1.0 mL-min-1 ;the mobile phase consisted of methanol-l% formic acid （40： 60） with the detection wavelength at 286 nm ; the column temperature was at 30 ℃. Result ： A good linear correlation of salvianolic acid B was observed within the range of （0. 207-8. 264） g （r =0. 999 9）, with the average recovery was 100.20% （RSD 1.14% ）. Conclusion： The method is simple and accurate with good specificity and reproducibility, and it can be used for the quality control and evaluation of Danxi Granula.