穿龙薯蓣HPLC指纹图谱及模式识别

HPLC Fingerprint Analysis and Pattern Recognition of Dioscorea nipponica

目的:建立穿龙薯蓣药材HPLC指纹图谱。方法:Agilent TC-C18(4.6 mm×250 mm,5μm)色谱柱;流动相乙腈(A)-水(B)梯度洗脱,检测波长203 nm,流速1.0 mL·min-1。收集了不同产地野生和栽培的28批样品进行测定,采用主成分分析筛选出12批样品,聚类分析结果与主成分分析结果相似,剔除1个距离较远的样品,最终筛选11批样品建立指纹图谱共有模式。结果:确立了14个共有峰,方法学考察结果良好,11批样品的相似度均>0.866。结论:该方法可用于穿龙薯蓣质量控制及综合评价。

Objective： To establish Dioscorea nipponica HPLC fingerprint. Method： The separation was performed on a Agilent TC-C18（4.6mm×250mm,5μm） column, with acetonitrile-water as mobile phase for gradient elution; the UV detector was set at 203 nm; the flow rate was 1.0 mL.min-1. There were 28 samples of wild and cultivated from various places to be analyzed. There were 12 samples selected by principal component analysis （PCA）, the result of cluster analysis was similar with PCA, except one sample which had been eliminated. 11 samples were selected to establish the common pattern of the fingerprint. Result： 14 marked common peaks were established, inspection results of the method were good, the similarities of 11 samples were more than 0. 866. Conclusion： The method provides an academic reference for the quality control of Dioscorea nipponica.