植物生长调节物质对白豆蔻花粉萌发和花粉管生长的影响

Effects of Plant Growth Regulating Substances on Pollen Germination and Tube Growth in Amomum kravanh

目的:为给杂交育种和人工辅助授粉提供参考。方法:用花粉离体培养法,研究了不同植物生长调节剂对泰国白豆蔻花粉萌发和花粉管生长的影响。结果:不同植物生长调节物质对花粉萌发和花粉管生长的作用不同。6-苄氨基嘌呤(6-BA)、赤霉素对白豆蔻花粉萌发及花粉管生长均表现较强的抑制作用;萘乙酸在0.5 mg·L-1时不仅有利于花粉萌发而且有利于花粉管生长;硼酸在100～200 mg·L-1质量浓度范围内促进白豆蔻花粉的萌发和花粉管的生长,最适质量浓度为100 mg·L-1;多效唑对白豆蔻花粉萌发起抑制作用,抑制程度随质量浓度的提高而减弱,促进白豆蔻花粉萌发时,可选择800 mg·L-1的多效唑质量浓度。结论:生产中在花期喷施一定浓度萘乙酸、硼酸或多效唑,可促进花粉萌发和花粉管生长,提高泰国白豆蔻的坐果率。

Objective： In order to guide cross breeding and artificial pollination. Method： We studied effects of plant growth regulators on pollen germination and tube growth in Amomum kravanh with pollen in vitro . Result： The results showed that： different plant growth regulators have a different effect on pollen germination and pollen tube growth in vivo . 6-benzylaminopurine （6-BA）, GA showed a strong inhibitory effect on pollen germination and pollen tube growth in all concentrations of the experiment in A. kravanh; naphthylacetate can promote the pollen germination and pollen tube growth at a concentration of 0.5 mg. L-1; the promotion concentration for boric acid is at 100-200 mg .L-1 range, the optimal concentration is 100 mg .L-1 paclobutrazol inhibits pollen germination in A. kravanh, inhibitory effect weakened with the increased of paclobutrazol concentration, 800 mg.L-1 concentration of paclobutrazol can promote pollen germination in A. kravanh. Conclusion： In the production practice, spraying a certain concentration of naphthyl acetic acid （NAA） , boric acid or paclobutrazol in the flowering period can promote pollen germination and pollen tube growth, and improve fruit set rate of Amomum Kravanh.