六味地黄生物制剂多糖对果蝇抗氧化作用的影响

Effect of Polysaccharides Extracting from Liuwei Dihuang Decoction Metabolized by Photosynthetic Bacteria on Antioxidative Ability of Drosophila melanogaster

目的:观察六味地黄生物制剂多糖(LW-PSB多糖)对果蝇抗氧化作用的影响。方法:采用美国野生型黑腹果蝇为实验对象,收集8 h内羽化未交配果蝇,雌雄分开,在温度为(25±1)℃,相对湿度为(60±5)%的人工气候箱中用培养基进行培养,将果蝇随机分为阴性对照组(普通培养基),阳性对照维生素C组(添加维生素C 1 g·kg-1的培养基),LW-PSB多糖低、中、高剂量组(含不同浓度的LW-PSB多糖的培养基,浓度依次为1,3,9 g·kg-1),共5组,每组雌、雄各500只。果蝇30 d龄时分别制成匀浆,测定果蝇体内超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-Px)、总抗氧化物(TAOC)活性及丙二醛(MDA)含量。结果:与阴性对照组比较,LW-PSB多糖各剂量组能提高果蝇SOD,CAT,GSH-Px和TAOC活性(P<0.05,P<0.01或P<0.001),降低MDA含量(P<0.01或P<0.001);与维生素C组比较,LW-PSB多糖各剂量组能不同程度地提高GSH-Px和TAOC活性(P<0.05或P<0.001),降低MDA含量(P<0.01或P<0.001)。结论:六味地黄生物制剂多糖饲喂果蝇可提高果蝇体内的抗氧化能力。

Objective： To investigate effect of polysaccharides extracting from Liuwei Dihuang decoction metabolized by photosynthetic bacteria （LW-PSB） on the antioxidative ability of Drosophila melanogaster. Method： Eight h eclosion unmated （D. melanegaster） were collected. Male and female were separated, cultivated in the artificial box with the culture medium at the temperature of （25 ± 1 ） ℃ , relative humidity for （60 ± 5） %. D. melanogaster were randomly divided into 5 groups ： negative control group, positive control group, LW-PSB polysaceharides groups （1, 3, 9 g.kg-1）. Thirty-day old D. melanogaster were made into homogenate and the liveness of superoxide dismutase （SOD）, catalase （CAT）, glutathione peroxidase （GSH-Px）, total antioxidant capacity （TAOC） and the malonaldehyde （MDA） content were detected. Result： Compared with the negative group, the antioxidase activity such as SOD, CAT, GSH-Px, TAOC of 30-day old D. melanogaster were significantly increased （P 〈 0. 05, P 〈 0.01 or P 〈 0. 001 ） and the MDA content was decreased （P 〈 0.01 or P 〈 0. 001 ）. Compared with the positive group, the antioxidase activity such as GSH-Px, TAOC of 30-day old D. melanogaster were Significantly increased （P 〈 0.05 or P 〈 0. 001 ） and the MDA content was decreased （P 〈 0.01 or P 〈0. 001）. Conclusion： LW-PSB polysaccharides might have a satisfactory effect on anti-oxidant.