活血化痰方治疗大鼠非酒精性脂肪肝

Effect of Huoxue Huatan Decoction on Indexes of Biochemistry in Experimental Model of Nonalcoholic Fatty Liver in Rats

目的:观察活血化痰方治疗大鼠非酒精性脂肪肝模型生化指标的影响。方法:以高脂饲料喂养6周建立非酒精性脂肪肝病大鼠模型,随机分为正常组,模型组,活血化痰方高、低剂量组,罗格列酮高、低剂量组6组,分别给予生理盐水10 mL·kg-1·d-1,活血化痰方生药38.5,19.25 g·kg-1·d-1,罗格列酮10,5 mg·kg-1·d-1,给药6周。实验结束时,检测大鼠基本生化指标,取肝脏组织进行病理检测。结果:活血化痰方高、低剂量组ALT水平明显降低,治疗效果优于罗格列酮和模型组[(50.7±12.2)(41.3±9.9)(66.3±29.1)(56.9±14.2)(71.3±25.9)U.L-1,P<0.05],活血化痰方高、低剂量组均能显著降低血清总胆固醇(TC)水平[(1.3±0.3)(1.5±0.4)(2.6±1.1)mmol·L-1,P<0.01]和甘油三酯(TG)水平[(0.3±0.2)(0.4±0.2)(0.5±0.2)mmol·L-1,P<0.05],高剂量组能明显降低血糖(GLU)水平[(9.0±1.3)(11.4±2.0)mmol·L-1,P<0.01],活血化痰方和罗格列酮显著改善血液流变学各项指标(P<0.05),且活血化痰方疗效整体优于罗格列酮组,活血化痰方高剂量组也可明显降低模型动物肝脏中TG[(0.73±0.16)(0.81±0.22)(0.57±0.11)(1.06±0.33)mmol·mg-1,P<0.05]和游离脂肪酸(FFA)[(1.77±0.29)(1.66±0.44)(2.04±0.27)μmol·mg-1,P<0.05]。结论:活血化痰方能够从改善肝功能、调节血脂代谢等多个方面改善非酒精性脂肪肝的病理状态。

Objective： To observe the curative effect of Huoxue Huatan decoction on experimental model of nonalcoholic fatty liver in rats. Method： Experimental animals model of nonalcoholic fatty liver in rats were established by feed of fatty food for 6 weeks, then experimental animals were divided into 6 groups which were treated with Huoxue Huatan decoction and Rosiglitazone respectively for another 6 weeks. Biochemical indexes and liver tissue of rats were tested after treatment. Result： Huoxue Huatan decoction could reduce the level of alanine aminotransferase （ALT）, which was much better than Rosiglitazone [ （50.7 ± 12.2）, （41.3 ±9.9）, （66.3 ± 29.1）, （56.9±14.2）, （71.3 ±25.9） U.L-1, P〈0.05], and Huoxue Huatan decoction can also reduce the level of total cholesterol （TC） [ （1.3 ±0.3）, （1.5 ±0.4）, （2.6 ± 1.1） mmol.L-1, P 〈0.01] and triglyceride （TG） [ （0.3 ±0. 2）, （0. 4 ±0.2）, （0.5 ±0. 2） mmol .L-1 , P 〈0. 05], while the high dose group of Huoxue Huatan decoction could reduce glucose （Glu） obviously [ （9.0 ± 1.3） （11.4 ± 2.0） mmol .L-1, p 〈 0.01 ] and improve the hemorheology indexes （P 〈0.05）, reduce the level of FFA [ （1.77 ±0. 29）, （1.66 ± 0.44）, （2.04±0.27） μmol.mg-1, P〈0.05] andTG [ （0.73 ±0.16）, （0.81 ±0.22）, （0.57±0.11）, （ 1.06 ± 0. 33） mmol mg-1, p 〈 0.05 ] in liver. Conclusion： Huoxue Huatan decoction ean improve pathological conditions of nonalcoholic fatty liver disease （NAFLD） model by adjusting metabolism of blood liquid and improving liver function.