芪参健骨颗粒对股骨头坏死大鼠脂代谢的影响

Effect of Qishen Jiangu Granula on Lipid Metabloismin Rats with Steroid-induced Avascular Necrosis of Femoral Head

目的:考察芪参健骨颗粒对激素性股骨头坏死大鼠脂代谢的影响。方法:雄性SD大鼠60只,随机分为6组,分别为正常组、模型组、芪参健骨颗粒低、中、高剂量给药组(15,30,60 g·kg-1)、阳性对照丹郁骨康丸组(3 g·kg-1),每组10只,除正常组外其余各组均用尾静脉注射脂多糖(20μg·kg-1)连续2 d后,肌肉注射甲基强的松龙20 mg·kg-1连续3 d复制股骨头坏死模型,每隔10 d称体重1次,预防性灌胃给药8周后取血清测定各组大鼠的胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白(HDL-C)和低密度脂蛋白(LDL-C)。取股骨标本,HE染色,光镜下观察骨组织病理改变。结果:与正常组比较,模型组大鼠体重增长缓慢,芪参健骨颗粒高、中、低剂量给药组能不同程度的抑制体重增长缓慢现象(P<0.01);与正常组比较,模型组大鼠TG,TC,LDL-C胆固醇含量增高(P<0.01和P<0.05),HDL-C胆固醇含量降低(P<0.05),给药组能明显抑制股骨头坏死出现脂代谢紊乱。结论:芪参健骨颗粒能明显改善激素性股骨头坏死模型的脂肪代谢紊乱,从而防治激素性股骨头坏死。

Objective： To study the effect of Qishen Jiangu granula （QSJGG） on lipid metabolism of avascular necrosis of the femoral head induced by hormone combining endotoxin. Method： Sixty SD rats were divided into 6 groups, respectively the control group, the model group, the low, medium, high-dose group （15, 30, 60 g. kg-1） of QSJGG, and the positive control group （3 g -kg-1）, and each group was replicated. Avascular necrosis of femoral head （ANFH） experimental animal model was made by muscle injection of methylprednisolone （20 mg .kg-1） for 3 days after injection of IOCV lipopolysaccharide （20 ug-kg-1） for 2 days in addition of the control group, after 8 week intragastric pre-administration of the medication, rats were sacrificedto collect blood from abdominal vein to determination of serum cholesterol （TC） , triglyceride （TG） , high density lipoprotein （HDL-C） and low density lipoprotein （LDL-C）, and femurs were obtained from both sides, of which the right side was used for pathological sections of HE staining to make observation of bone tissue. Result： To Compared with the model normal rats, fat cells of medullary cavity in the QSJGG group was more and relativity big （P 〈0. 01）, TC, TG and LDL-C leels were increased （P 〈0.01, P 〈0.05） ,HDL-C was reduced （P 〈0.05）. Compared with group, fat cells of medullary cavity in the model group was less and relativity small, TC, TG and LDL-C were reduced, HDL-C was increased. Conclusion： QSJGG can significantly improve fat metabolism of model of femoral head necrosis, which control femoral head necrosis.