穿破石水提取物对四氯化碳致大鼠肝纤维化的治疗作用

Effect of Water Extract of Cudrania cochinchinensis on Liver Fibrosis Rat Models Induced by Carbon Tetrachloride

目的:研究穿破石水提取物对大鼠肝纤维化的治疗作用。方法:SD大鼠按体重随机分为对照组、模型组、阳性药秋水仙碱组(0.000 25 g·kg-1)和穿破石水提取物高剂量组(4.57 g·kg-1)、中剂量组(3.05 g·kg-1)、低剂量组(1.52 g·kg-1)。除对照组外,其余各组大鼠均皮下注射40%四氯化碳(CCl4)橄榄油液3 mL·kg-1(首次为5 mL·kg-1)每周2次,连续8周,造模同时各给药组给药。8周后测定丙氨酸氨基转移酶(ALT),天冬氨酸转氨酶(AST),总蛋白质(TP),白蛋白(Alb),球蛋白(Glob),血清一氧化氮(NO)、丙二醛(MDA)、超氧化物歧化酶(SOD)、碱性磷酸酶(AKP)和肝纤维化指标透明质酸(HA)、层粘连蛋白(LN)、Ⅲ型前胶原(PCⅢ)、Ⅳ型胶原(CⅣ);取肝制备肝匀浆,测定羟脯氨酸(HYP)水平,并取肝组织做病理检查。结果:大鼠肝纤维化损伤时,模型组ALT,AST活性及血清AKP,HA,LN和CⅣ含量及肝组织HYP明显增加,而TP,Alb,SOD明显降低,与对照组比较差异有显著性(P<0.05);与模型组比较,穿破石水提取物可降低ALT,AST活性及血清PCⅢ,HA含量及肝组织HYP水平,而提高血清SOD,TP,ALB含量,差异均有显著性(P<0.05)。病理学检查结果显示,穿破石水提取物治疗组肝纤维化有显著改善。结论:穿破石水提取物对四氯化碳所致的大鼠肝纤维化具有良好的治疗作用。

Objective： To investigate the anti-liver fibrosis effect of water extract of Cudrania cochin- chinensis in rats. Method： One hundred and thirty Sprague-Dauley （SD） rats were randomly divided into control group, model group, postitive control group and three differrent doses （4.57 g.kg-1, 3.05 g.kg-1. 1.52 g.kg-1） of water extract of C. cochinchinensis treatment group. Liver fibrosis rat model was induced by carbon tetrachloride （CC14）. Except control group, other groups were given drugs when being given carbon tetrachloride. Ending the study at the eighth week, liver function （alanine amino transferase （ALT）, aspartate amino transferase （AST） , total protein （ TP）, albumin （Alb） , globulin （Glob） ） , serum concentrations of nitric oxide （ NO ） , malondialdehyde （MDA）, alkaline phosphatase （AKP）, hyaluronic acid （HA）, laminin （LN）, procollagen- III（ PC 111 ） and collageri-IV （C 1V ） were measured. The concentrations of hydroxyproline （HYP） in liver hydrolysate were measured too. Moreover, pathological examination on liver tissue was made. Result： Serum concentrations of ALT, AST, AKP, HA, LN, C1V and concentration of HYP in liver hydrolysate was significantly higher in liver fibrosis model group, however, the concentrations of TP, Alb, SOD were significantly lower in model group. Compared with model group, the serum concentrations of ALT, AST, PC III, HA and concentration of HYP in liver hydrolysate were significantly decreased in water extract of C. cochinchinensis treatment groups and the serum concentrations of SOD, TP, Alb were significantly increased in water extract of C. cochinchinensis treatment groups. Pathological result showed that the liver fibrosis in water extract of C. cochinchinensis groups was significant improved. Conclusion： Water extract of C. cochinchinensis has significant therapeutic effect on liver fibrosis induced by carbon tetrachloride in rats.