德都红花-7味散对CCl_4诱导实验性肝纤维化治疗作用

Experimental Treatment of Deduhonghua-7 Powder on CCl_4 Induced Hepatic Fibrosis

目的:观察德都红花-7味散对四氯化碳(CCl4)所致大鼠肝纤维化的治疗作用。方法:将大鼠按体重随机分为空白对照组、模型组、秋水仙碱组、德都红花-7味散组。除空白对照组,其他组ip 30%CCl4橄榄油溶液诱导大鼠肝纤维化。造模同时德都红花-7味散组和秋水仙碱组分别ig德都红花-7味散和秋水仙碱每日0.62 g·kg-1和0.4 mg·kg-1,连续7周。观察肝脏系数、肝功能、纤维4项,用HE,Gomori,Masson 3种方法染色,观察肝组织病理学改变。结果:与空白对照组比较,模型组丙氨酸氨基转氨酶(ALT)、白蛋白(Alb)、总蛋白(TP)、总胆红素(TBIL)、碱性磷酸酶(ALP)等,肝脏指数、血清及肝组织匀浆纤维4项明显升高,Alb和体重降低,P<0.05。与模型组比较,德都红花-7味散组AST,ALT,ALP,肝脏指数,肝组织匀浆Hyp,血清和肝组织纤维4项明显降低,Alb升高,秋水仙碱组ALT,AST,ALP,BIL降低,肝组织HA升高,Ⅳ降低(P<0.05)。与秋水仙碱组比较德都红花-7味散组Alb含量升高,血清HA,LN含量降低,肝组织HA,LN,ⅣC,Hyp含量降低(P<0.05)。结论:德都红花-7味散有较好的降酶保肝、抗肝纤维化作用。

Objective： To observe the treatment of Deduhonghua-7 Powder on rat with carbon tetrachloride （CC14） induced hepatic fibrosis. Method： Rats were randomly divided into normal control group, model group, Colchicine group and Deduhonghua-7 powder group. Except the normal control group, other groups were caused hepatic fibrosis by ip 30% CC14 solution of olive oil. Modeling the same time, the rats in Deduhonghua-7 powder group and Colchicine group were ig 0.62 g.kg-1 and 0.4 mg.kg-1 each day of Deduhonghua-7 powder and Colchine respectively, continued for 7 days. Liver indexes, liver function, fibrosis four projects were observed, pathological changes in liver tissues were evaluated by HE, Gomori, Masson stainings. Result： Comparing with normal control group, aspartate transaminase （AST）, alanine transpeptidase （ALT）, alkaline phosphatase （ALP）, total protein （TP）, total bilirubin （TBIL）, liver indexes, fibrosis four projects of serum and liver tissues homogenates were significantly increased, albumin （Alb） , and bodyweight were decreased in the model group （P 〈 0. 05）. Comparing with model group, in the Deduhonghua-7 Powder group, AST, ALT, ALP, liver indexes, liver tissues homogenates Hyp, serum and liver tissues fibrosis four projects were significantly decreased, Alb was increased; and in the Colchine group, ALT, AST, ALP, BIL were decreased, liver tissues hyaluronic acid （HA） was increased, IV was decreased （P 〈 0. 05 ）. Comparing with Colchine group, Deduhonghua-7 Powder group＇ s Alb was increased, serum HA, LN contents were decreased, liver tissues HA, LN, IV-C, Hyp contents were decreased （P 〈 0.05）. Conclusion： Deduhonghua-7 Powder showed good effect on reducing enzyme, protecting liver and anti-fibrosis for liver.