祛湿清热方对湿热模型的作用

Effect of Qushi Qingre Decoction on Model of Dampness-heat Syndrome

目的:评价祛湿清热方对湿热模型的治疗作用。方法:采用多因素致湿热证动物模型,将SD大鼠饲以高糖高脂饲料,高温高湿环境下培养,定期ig以鼠伤寒沙门氏菌进行感染,检测以3.6,1.8,0.9 g·kg-1祛湿清热方ig连续7 d对模型动物全血黏度、血液和皮肤超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量的影响,研究祛湿清热方对改善湿热证的药效学作用。结果:清热祛湿方高、中剂量组,高、中、低切变率下的全血黏度均明显下降(P<0.01,P<0.05),高剂量组效果最好,高、中、低切变率下全血黏度分别为(2.43±0.22),(2.88±0.19),(8.07±0.75)mPa.s,明显低于模型组(2.92±0.20),(3.26±0.25),(9.88±0.63)mPa.s;高、中剂量组血清SOD活性分别为(6.16±1.12),(4.05±1.54)U.L-1明显高于模型组(2.67±1.04)U.L-1(P<0.01),高、中、低剂量血清MDA含量为(3.78±1.18),(4.56±0.96),(7.39±1.79)mmol·L-1明显低于模型组(9.15±1.72)mmol·L-1(P<0.01,P<0.05);高、中、低剂量组大鼠皮肤MDA水平分别为(0.65±0.23),(0.84±0.20),(1.04±0.35)mmol·L-1,明显低于模型组(1.58±0.51)mmol·L-1(P<0.01,P<0.05);高剂量组皮肤SOD(1.42±0.41)U.L-1明显高于模型组(1.00±0.26)mmol·L-1(P<0.01)。结论:清热祛湿方可以使湿热模型的生理指标明显得到改善。

Objective： To evaluate influence of Qushi Qingre decoction on model of Dampness-heat syndrome. Method： The model of dampness-heat syndrome was induced by multi-factors, including feeding high sugar and high fat food, keeping in an environment, with high temperature and humidity, and infection of rat Salmonella typhi periodically. The effect of Qushi Qingre decoction （3.6, 1.8, 0.9 g .kg-1, ig 7 d） on dampness- heat syndrome was investigated by whole blood viscosity and the activity of superoside dismutase （SOD） and the malondialdehyde （MDA） in the model rats. Result： Qushi Qingre decoction high dose and middle dose groups could significantly reduce whole blood viscosity （ P 〈 0.01, P 〈 0.05 ） , and could significantly reduce the concentration of the blood and skin MDA （P 〈 0.01, P 〈 0.05）. High dose group showed the best effect, of blood viscosity at high, medium, low rates was respectively （2.43 ±0.22） , （2.88 ±0. 19）, （8.07 ±0.75） mPa ,s, significantly lower than those in the model group （2.92 ± 0.20） , （3.26 ± 0. 25 ） , （9.88 ± 0.63 ） mPa＇s. Qushi Qingre decoction high dose could significantly increase SOD activity in blood and skin （P 〈 0.01, P 〈 0.05 ） , SOD in high-dose group was （6. 16 ± 1.12）, （4.05 ± 1.54） U .L-lrespectively, significantly higher than that in the model group （2.67 ±1.04） U-L-1 1.18）, （4.56±0.96）, （7.39±1.79） 1.72） mmol-L-1. The skin MDA levels 0.20） , （1.04 ±0.35） mmol L-1 respec model group. Conclusion ： Qushi Qingre significantly. [ Key words] In the high, medium and low doses groups, the serum MDA （3.78 ± mmol .L-1 was obviously lower than those in the model group （9. 15 ± in the high, medium and low dose groups were （0. 65 ± 0.23） （0. 84 ：t： tively, significantly lower than that [ （1.58±0.51） mmol.L-1 ] in the decoction improves the parameters of the model of damp-heat syndrome significantly.